Emergency granulopoiesis occurs in response to severe microbial infection. However, whether and how other blood components, particularly monocytes/macrophages and their progenitors, including hematopoietic stem/progenitor cells (HSPCs), participate in the process and the underlying molecular mechanisms remain unknown. In this study, we challenged zebrafish larvae via direct injection of Escherichia coli into the bloodstream, which resulted in systemic inoculation with this microbe. The reaction of hematopoietic cells, including HSPCs, in the caudal hematopoietic tissue was carefully analysed. Both macrophages and neutrophils clearly expanded following the challenge. Thus, emergency myelopoiesis, including monopoiesis and granulopoiesis, occurred following systemic bacterial infection. The HSPC reaction was dependent on the bacterial burden, manifesting as a slight increase under low burden, but an obvious reduction following the administration of an excessive volume of bacteria. Pu.1 was important for the effective elimination of the microbes to prevent excessive HSPC apoptosis in response to stress. Moreover, Pu.1 played different roles in steady and emergency monopoiesis. Although Pu.1 was essential for normal macrophage development, it played suppressive roles in emergency monopoiesis. Overall, our study established a systemic bacterial infection model that led to emergency myelopoiesis, thereby improving our understanding of the function of Pu.1 in this scenario.
Myeloid phagocytes, neutrophils in particular, are easily consumed when they fight against a large number of invading microbes. Hence, they require efficient and constant replenishment from their progenitors via the well-orchestrated emergency myelopoiesis in the hematopoietic organs. The cellular and molecular details of the danger-sensing and warning processes to activate the emergency myelopoiesis are still under debate. In this study, we set up a systemic infection model in zebrafish (Danio rerio) larvae via circulative administration of LPS. We focused on the cross-talk of macrophages with myeloid progenitors in the caudal hematopoietic tissue. We revealed that macrophages first detected LPS and sent out the emergency message via il1b. The myeloid progenitors, rather than hematopoietic stem and progenitor cells, responded and fulfilled the demand to adapt myeloid expansion through the synergistic cooperation of NF-kB and C/ebpb. Our study unveiled a critical role of macrophages as the early "whistle blowers" to initiate emergency myelopoiesis.
SUMMARYThis paper presents a new type of forceps that consist of two microgrippers with the capability of direct force sensing, which enables grasping and manipulating forces at the tip of surgical instrument for minimally invasive robotic surgery. For the prototype design of the forceps, a double E-type vertical elastomer with four strain beams is presented, whose force-sensing principle is expounded. Thus, the forceps with the elastomer can be considered a compliant component, which provides tiny displacements that allow large strain, and the overall diameter is 10 mm. The sizes of the elastomer and forceps are successively determined by analyzing the relationship of several parameters and strain. Then, the linearity analysis of strain beams determines the positions to apply gauges for sensing. The two-dimensional force decoupling models for a single microgripper are proposed based on piecewise analytical polynomials of the strain difference and employed to develop a new three-dimensional force nonlinear decoupling algorithm based on double microgrippers, which realizes single-axial grasping and three-axial pulling forces. Finally, the required force-sensing performance of the proposed method is successfully verified in theory using finite-element simulations.
Genes whose dysfunction do not affect normal survival is a common phenomenon. Are they only meaningless residues during evolution? Here, we identified a fish-specific new gene, which we named lg. Gene knockout resulted in no obvious phenotype in zebrafish, but lg evolves an amino acid mutation that is under positive selection in the modern haplochromine (MH) cichlid fish lineage, the well-known adaptive radiative lineage. Moreover, the cichlid fish-specific upstream region of lg can drive new eGFP expression profiles in tissues related to adaptation. Besides, this homologous region from different cichlid fishes drive different eGFP expressions which is simply due to three MH-segregated SNPs mutations that are predicted to bind a hormone related transcription factor. We thus revealed an initially redundant gene evolving new functions in an adaptive radiative lineage. This further illuminates the mechanism about the emergence of new gene function with respect to evo-devo in a broad way.
Research on methane and carbon dioxide emissions mainly focuses on industrial emissions, cultivated land, and wetlands, while few studies have studied freezing-related emissions. This paper presents field experiments conducted during soil freezing to measure carbon dioxide and methane concentrations in the air, near the soil surface, and in the soil. In addition, the influence of precipitation, snowfall, air temperature, and depth of freezing on gas emissions was analyzed. We observed increased concentrations of methane and carbon dioxide in soil and air at soil freezing and snow cover growth. For the first time, an increase in gas flux during soil freezing was found in the absence of permafrost.
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