Background Non-alcoholic fatty liver disease (NAFLD) has become a global epidemic disease. Its incidence is associated with type 2 diabetes mellitus (T2DM). Presently, there is no approved pharmacological agents specially developed for NAFLD. One promising disease-modifying strategy is the transplantation of stem cells to promote metabolic regulation and repair of injury. Method In this study, a T2DM model was established through 28-week high-fat diet (HFD) feeding resulting in T2DM-associated NAFLD, followed by the injection of bone marrow mesenchymal stem cells (BMSCs). The morphology, function, and transfer of hepatocyte mitochondria were evaluated in both vivo and in vitro. Results BMSC implantation resulted in the considerable recovery of increasing weight, HFD-induced steatosis, liver function, and disordered glucose and lipid metabolism. The treatment with BMSC transplantation was accompanied by reduced fat accumulation. Moreover, mitochondrial transfer was observed in both vivo and vitro studies. And the mitochondria-recipient steatotic cells exhibited significantly enhanced OXPHOS activity, ATP production, and mitochondrial membrane potential, and reduced reactive oxygen species levels, which were not achieved by the blocking of mitochondrial transfer. Conclusion Mitochondrial transfer from BMSCs is a feasible process to combat NAFLD via rescuing dysfunction mitochondria, and has a promising therapeutic effect on metabolism-related diseases.

Abstract:Ion-sieves are a class of green adsorbent for extraction Li + from salt lakes. Here, we propose a facile synthesis of hexagonal spinel LiMn 2 O 4 (LMO) precursor under mild condition which was first prepared via a modified one-pot reduction hydrothermal method using KMnO 4 and ethanol. Subsequently, the stable spinel structured λ-MnO 2 (HMO) were prepared by acidification of LMO. The as-prepared HMO shows a unique hexagonal shape and can be used for rapid adsorption-desorption process for Li + adsorption. It was found that Li + adsorption capacity of HMO was 24.7 mg·g −1 in Li + solution and the HMO also has a stable structure with manganese dissolution loss ratio of 3.9% during desorption process. Moreover, the lithium selectivity (α Li Mg ) reaches to 1.35 × 10 3 in brine and the distribution coefficients (K d ) of Li + is much greater than that of Mg 2+ . The results implied that HMO can be used in extract lithium from brine or seawater containing high ratio of magnesium and lithium.

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