We rationally engineered an elegant entropy-driven DNA nanomachine with three-dimensional track and applied it for intracellular miRNAs imaging. The proposed nanomachine is activated by target miRNA binding to drive a walking leg tethered to gold nanoparticle with a high density of DNA substrates. The autonomous and progressive walk on the DNA track via the entropy-driven catalytic reaction of intramolecular toehold-mediated strand migration leads to continuous disassembly of DNA substrates, accompanied by the recovery of fluorescence signal due to the specific release of a dye-labeled substrate from DNA track. Our nanomachine outperforms the conventional intermolecular reaction-based gold nanoparticle design in the context of an improved sensitivity and kinetics, attributed to the enhanced local effective concentrations of working DNA components from the proximity-induced intramolecular reaction. Moreover, the nanomachine was applied for miRNA imaging inside living cells.
We rationally engineered an elegant entropy-driven DNAn anomachine with three-dimensional tracka nd applied it for intracellular miRNAs imaging.T he proposed nanomachine is activated by target miRNAb inding to drive awalking leg tethered to gold nanoparticle with ahigh density of DNAsubstrates.The autonomous and progressive walk on the DNAt rack via the entropy-driven catalytic reaction of intramolecular toehold-mediated strand migration leads to continuous disassembly of DNAs ubstrates,a ccompanied by the recovery of fluorescence signal due to the specific release of ad ye-labeled substrate from DNAt rack. Our nanomachine outperforms the conventional intermolecular reaction-based gold nanoparticle design in the context of an improved sensitivity and kinetics,a ttributed to the enhanced local effective concentrations of working DNAc omponents from the proximity-induced intramolecular reaction. Moreover,t he nanomachine was applied for miRNAi maging inside living cells.
Herein we utilized the thermal hysteresis method to directly probe the self-assembly process of amphiphilic DNA nanostructures, with the use of amphiphilic tetrahedral DNA framework (am-TDF) as a model system....
Herein we examined the utilization of orthogonal chemical interaction to program the self-assembly of amphiphilic DNA frameworks (am-FNA). By finely controlling the reaction parameters such as ionic strength, the length...
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