2017
DOI: 10.1002/anie.201704147
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Rational Engineering of a Dynamic, Entropy‐Driven DNA Nanomachine for Intracellular MicroRNA Imaging

Abstract: We rationally engineered an elegant entropy-driven DNA nanomachine with three-dimensional track and applied it for intracellular miRNAs imaging. The proposed nanomachine is activated by target miRNA binding to drive a walking leg tethered to gold nanoparticle with a high density of DNA substrates. The autonomous and progressive walk on the DNA track via the entropy-driven catalytic reaction of intramolecular toehold-mediated strand migration leads to continuous disassembly of DNA substrates, accompanied by the… Show more

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Cited by 347 publications
(202 citation statements)
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References 27 publications
(23 reference statements)
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“…Based on the similar principle, various DNA nanomachines have also been developed for the analysis of biological samples by designing different nucleic acid strands. Entropy‐ Driven DNA walker is a kind of nanomachine that has been developed for intracellular miRNA detection . They built three‐dimensional DNA tracks and capture probes on the surface of AuNPs.…”
Section: Hcc‐associated Mirna Detection Based On Enzyme‐free Isothermmentioning
confidence: 99%
“…Based on the similar principle, various DNA nanomachines have also been developed for the analysis of biological samples by designing different nucleic acid strands. Entropy‐ Driven DNA walker is a kind of nanomachine that has been developed for intracellular miRNA detection . They built three‐dimensional DNA tracks and capture probes on the surface of AuNPs.…”
Section: Hcc‐associated Mirna Detection Based On Enzyme‐free Isothermmentioning
confidence: 99%
“…Considering the complex and distinct intracellular environment of different cells, as well as the inhomogeneous uptake of DNA probes, here the FRET readout was acquired through the fluorescence emission ratio of acceptor to donor ( F A / F D ). Thus the hMNS/ACD system enables more reliable intracellular microRNA imaging without undesired fluctuations of different living cells, for example, high miR‐21‐expressing human breast cancer cells (MCF‐7), low miR‐21‐expressing human cervical cancer cells (HeLa), and no miR‐21‐expressing human embryonic lung fibroblast cells (MRC‐5) . After the hMNS/ACD imaging system was incubated with these living cells for approximately 4 h (an optimized incubation time, see Figure S10 in the Supporting Information), a more intensive FRET signal was observed in MCF‐7 cells (Sample a, Figure C) in comparison with that in HeLa cells (Sample d, Figure C).…”
Section: Resultsmentioning
confidence: 99%
“…Other design variations have sought to add signal amplification to NanoFlares. Liang et al recently developed an entropy driven amplifier in conjunction with conventional NanoFlares, reaching a detection limit of 8 p m in solution and imaging miR‐21 in MCF‐7, HeLa, HEK293, and MRC‐5 cells . Moreover, Li et al recently interfaced FRET NanoFlares with an amplification element for sensitive miRNA detection .…”
Section: Hybridization‐based Probesmentioning
confidence: 99%