The potential of the diverse chemistries present in natural products (NP) for biotechnology and medicine remains untapped because NP databases are not searchable with raw data and the NP community has no way to share data other than in published papers. Although mass spectrometry techniques are well-suited to high-throughput characterization of natural products, there is a pressing need for an infrastructure to enable sharing and curation of data. We present Global Natural Products Social molecular networking (GNPS, http://gnps.ucsd.edu), an open-access knowledge base for community wide organization and sharing of raw, processed or identified tandem mass (MS/MS) spectrometry data. In GNPS crowdsourced curation of freely available community-wide reference MS libraries will underpin improved annotations. Data-driven social-networking should facilitate identification of spectra and foster collaborations. We also introduce the concept of ‘living data’ through continuous reanalysis of deposited data.
Green fluorescence protein (GFP), which serves as an energy acceptor and emitter for bioluminescence in the sea pansy Renilla reniformis and the jellyfish Aequorea Victoria, has drawn much attention because of its applications in molecular biology and biochemistry. 1 GFP takes advantage of the presence of a chromophore that is anchored both covalently and via a hydrogen-bond network, 4-(4-hydroxybenzylidene)-1,2-dimethyl-1H-imidazol-5(4H)-one (p-HBDI, see Scheme 1), which undergoes excited-state proton transfer (ESPT) 2 via the proton relay of water molecules and a remote residue such as E222, 3 resulting in a very effective and intense anion fluorescence.Nevertheless, studies reveal a strong cutoff between the properties of wild type GFP (or certain GFP mutants) and the synthetic analogue chromophores of p-HBDI. 4 In view of photophysics, the fluorescence yield of the protein-free chromophore in fluid solvents is much weaker and strongly temperature dependent. The results suggest an efficient radiationless transition operating in p-HBDI, most probably induced by conformational relaxation along torsional deformation of the two exocyclic C-C bonds to a nonfluorescent twisted intermediate. 5 More recently, it has been proposed that the shallow potential energy surface of the intermediates may conically intersect with that of the ground state, inducing the dominant radiationless deactivation. 4c-d,6 Such a conformational relaxation is greatly suppressed in GFP by its proton relay, rigid environment.In view of chemistry, most of the research has been focused on the chemical modification of p-HBDI analogues at the C(1) position. 4c,7 Conversely, in this study, we are interested in the derivatization on the phenyl ring. As an ingenious approach, switching the hydroxyl group from the C(8) position to the C(6) position (see Scheme 1), forming 4-(2-hydroxybenzylidene)-1,2-dimethyl-1H-imidazol-5(4H)-one (o-HBDI), a structural isomer of p-HBDI may reveal several novel features with respect to p-HBDI. The geometry optimization (B3LYP/cc-pVDZ and aug-cc-pVDZ, see ESI) of o-HBDI unveils the existence of a seven-memberedring intramolecular hydrogen bond between -OH and the N(2) atom. This intramolecular hydrogen-bonding configuration should, in part, hinder the exocyclic torsional deformation such that the radiationless deactivation may be reduced. More importantly, theoretical approaches also predict that excited-state intramolecular proton transfer (ESIPT) from the OH proton to the N(2) atom is thermally favorable (vide infra), forming a zwitterionic tautomer species (see Table of Content, TOC).In light of these perspectives, we have thus expended great effort to make a facile synthesis of o-HBDI. Briefly, the o-methoxybenzaldehyde was used as a starting reactant (see Scheme 1). Because of the lack of the o-hydroxyl group and hence the intramolecular lactonation, 3 was obtained with a good yield (70%). Subsequent reaction of 3 with methylamine, followed by deprotection of the methyl group of o-MBDI by BBr 3 , afforded o-H...
A series of newly synthesized Os(II) and Ag(I) complexes exhibit remarkable ratiometric changes of intensity for phosphorescence versus fluorescence that are excitation wavelength dependent. This phenomenon is in stark contrast to what is commonly observed in condensed phase photophysics. While the singlet to triplet intersystem crossing (ISC) for the titled complexes is anomalously slow, approaching several hundred picoseconds in the lowest electronic excited state (S(1) → T(1)), higher electronic excitation leads to a much accelerated rate of ISC (10(11)-10(12) s(-1)), which is competitive with internal conversion and/or vibrational relaxation, as commonly observed in heavy transition metal complexes. The mechanism is rationalized by negligible metal d orbital contribution in the S(1) state for the titled complexes. Conversely, significant ligand-to-metal charge transfer character in higher-lying excited states greatly enhances spin-orbit coupling and hence the ISC rate. The net result is to harvest high electronically excited energy toward triplet states, enhancing the phosphorescence.
Mass spectrometry imaging (MSI) using ambient ionization technique enables a direct chemical investigation of biological samples with minimal sample pretreatment. However, detailed morphological information of the sample is often lost due to its limited spatial resolution. In this study, predictive high-resolution molecular imaging was produced by the fusion of ambient ionization MSI with optical microscopy of routine hematoxylin and eosin (H&E) staining produces. Specifically, desorption electrospray ionization (DESI) and nanospray desorption electrospray ionization (nanoDESI) mass spectrometry are employed to visualize lipid and protein species on mice tissue sections. The resulting molecular
Cisplatin is a potent anti-cancer drug, however, its accompanied organ-toxicity hampers its clinical applications. Cisplatin-associated kidney injury is known to result from its accumulation in the renal tubule with excessive generation of reactive oxygen species. In this study, we encapsulated honokiol, a natural lipophilic polyphenol constituent extracted from Magnolia officinalis into nano-sized liposomes (nanosome honokiol) and examined the in vivo countering effects on cisplatin-induced renal injury. We observed that 5 mg/kg body weight. nanosome honokiol was the lowest effective dosage to efficiently restore renal functions of cisplatin-treated animals. The improvement is likely due the maintenance of cellular localization of cytochrome c and thus preserves mitochondria integrity and their redox activity, which as a consequence, reduced cellular oxidative stress and caspase 3-associated apoptosis. These improvements at the cellular level are later reflected on the observed reduction of kidney inflammation and fibrosis. In agreement with our earlier in vitro study showing protective effects of honokiol on kidney cell lines, we demonstrated further in the current study, that nanosuspension-formulated honokiol provides protective effects against cisplatin-induced chronic kidney damages in vivo. Our findings not only benefit cisplatin-receiving patients with reduced renal side effects, but also provide potential alternative and synergic solutions to improve clinical safety and efficacy of cisplatin treatment on cancer patients.
Imaging mass spectrometry (IMS) is a powerful technique that enables analysis of various molecular species at a high spatial resolution with low detection limits. In contrast to the matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) approach, surface-assisted laser desorption/ionization (SALDI) can be more effective in the detection of small molecules due to the absence of interfering background signals in low m/z ranges. We developed a functionalized TiO 2 nanowire as a solid substrate for IMS of low-molecular-weight species in plant tissues. We prepared TiO 2 nanowires using an inexpensive modified hydrothermal process and subsequently functionalized them chemically with various silane analogs to overcome the problem of superhydrophilicity of the substrate. Chemical modification changed the selectivity of imprinting of samples deposited on the substrate surface and thus improved the detection limits. The substrate was applied to image distribution of the metabolites in very fragile specimens such as the petal of Catharanthus roseus. We observed that the metabolites are distributed heterogeneously in the petal, which is consistent with previous results reported for the C. roseus plant leaf and stem. The intermediates corresponding to the biosynthesis pathway of some vinca alkaloids were clearly shown in the petal. We also performed profiling of petals from five different cultivars of C. roseus plant. We verified the semi-quantitative capabilities of the imprinting/imaging approach by comparing results using the LC-MS analysis of the plant extracts. This suggested that the functionalized TiO 2 nanowire substrate-based SALDI is a powerful technique complementary to MALDI-MS.
A series of novel anthranilic acid derivatives I–IV, of which COOH-NH2 (I) and COOH-NHMe (IV) are endowed with acid and base bifunctionality, were designed and synthesized for matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry applications in dual polarity molecular imaging of biological samples, particularly for lipids. The heat of protonation, deprotonation, and proton transfer reaction as well as the capability of analyzing biomolecules in both positive and negative ion modes for I–IV were systematically investigated under standard 355 nm laser excitation. The results indicate correlation between dual polarity and acid–base property. Further, COOH-NHMe (IV) showed a unique performance and was successfully applied as the matrix for MALDI-TOF mass spectrometry imaging (MSI) for studying the mouse brain. Our results demonstrate the superiority of COOH-NHMe (IV) in detecting more lipid and protein species compared to commercially available matrices. Moreover, MALDI-TOF MSI results were obtained for lipid distributions, making COOH-NHMe (IV) a potential next generation universal matrix.
Triosmium cluster complexes [Os3(CO)8(fppz)2] (2a) and [Os3(CO)8(fptz)2] (2b) bearing two 2-pyridyl azolate ligands were synthesized in an attempt to establish the reaction mechanism that gives rise to the blue-emitting phosphorescent complexes [Os(CO)2(fppz)2] (1a) and [Os(CO)2(fptz)2] (1b) [(fppz)H = 3-(trifluoromethyl)-5-(2-pyridyl)pyrazole; (fptz)H = 3-(trifluoromethyl)-5-(2-pyridyl)triazole]. X-ray structural analysis of 2b showed an open triangular metal framework incorporating multisite-coordinated 2-pyridyltriazolate ligands. Treatment of 2 with the respective 2-pyridylazolate ligand led to the formation of blue-emitting complex 1b, confirming their intermediacy, while the reaction of 2b with phosphine ligand PPh2Me afforded two hitherto novel hydride complexes 3 and 4, for which the reversible interconversion was clearly established at higher temperatures (> 180 degrees C). The single-crystal X-ray diffraction analyses of 3 and 4 confirmed their monometallic and isomeric nature, together with the coordination of two phosphine ligands located in the trans-disposition and one CO and one hydride located opposite to the pyridyl triazolate chelate. Subtle differences in photophysical properties were examined for isomers 3 and 4 on the basis of steady state absorption and emission, the relaxation dynamics, and temperature-dependent luminescent studies. The results, in combination with time-dependent density function theory (TDDFT) calculations, provide fundamental insights into the future design and preparation of highly efficient phosphorescent emitters.
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