Carbon-13 nuclear magnetic resonance studies were made on mouse hemoglobin specifically labeled at the C-2 histidine position. Measurement of the spin lattice relaxation times of the label before and after hemolysis of the erythrocytes provides information on the intracellular fluid viscosities.
The time course study of the utilization of specifically labeled glucose by yeast has been carried out under proliferating conditions. The rate of change of specific activity in the respiratory COs from the respectively labeled substrate agrees with the known breakdown pathways of glucose in this organism. Calculations were made on the basis of cumulative radiochemical recoveries of metabolic CO, from each labeled glucose which revealed that approximately 87% of the administered glucose was metabolized by way of the Embden-Meyerhof pathway in combination with the tricarboxylic acid cycle, and 13% was consumed through phosphogluconate decarboxylation. The efficiency of the utilization of each glucose carbon atom in biosynthesis also was estimated.
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