“…This is very much higher than that reported for isolated housefly sarcosomes. The highest value measured manometrically is repbrted by Gregg, Heisler & Remmert (1960), who found, with pyruvate plus fumarate as substrates, QO, 114 at 25°. Sacktor & Cochran (1958), in manometric studies, obtained the highest respiratory rates when o-glycerophosphate was the substrate (QO 55).…”
mentioning
confidence: 96%
“…The lack of a measurable response of respiration of their isolated housefly sarcosomes to the addition of phosphate (Sacktor, 1954) or adenosine diphosphate (Sacktor & Cochran, 1958) led Chance & Sacktor (1958) and Estabrook & Sacktor (1958) to the proposals that the respiratory rate is controlled by the substrate concentration or by selective inhibition of certain oxidase systems. However, dependence of the rate of respiration of isolated insect sarcosomes on the concentration of adenosine diphosphate has now been firmly established (Gonda, Traub & Avi-Dor, 1957;Klingenberg & Bucher, 1959; Gregg et al 1960).…”
“…This is very much higher than that reported for isolated housefly sarcosomes. The highest value measured manometrically is repbrted by Gregg, Heisler & Remmert (1960), who found, with pyruvate plus fumarate as substrates, QO, 114 at 25°. Sacktor & Cochran (1958), in manometric studies, obtained the highest respiratory rates when o-glycerophosphate was the substrate (QO 55).…”
mentioning
confidence: 96%
“…The lack of a measurable response of respiration of their isolated housefly sarcosomes to the addition of phosphate (Sacktor, 1954) or adenosine diphosphate (Sacktor & Cochran, 1958) led Chance & Sacktor (1958) and Estabrook & Sacktor (1958) to the proposals that the respiratory rate is controlled by the substrate concentration or by selective inhibition of certain oxidase systems. However, dependence of the rate of respiration of isolated insect sarcosomes on the concentration of adenosine diphosphate has now been firmly established (Gonda, Traub & Avi-Dor, 1957;Klingenberg & Bucher, 1959; Gregg et al 1960).…”
“…It has been shown by Van den Bergh & Slater (1962) that pyruvate is probably the most important physiological substrate for respiration and energy production in housefly flight-muscle mitochondria (sarcosomes). Isolated sarcosomes rapidly oxidize added pyruvate (Gregg, Heisler & Remmert, 1960), and this oxidation is accompanied by a rapid esterification of Pi and is under the control of the ADP and Pi concentrations. Evidence was presented that the low respiratory rates found with most substrates (e.g.…”
“…Oxidative phosphorylation in insect mitochondria has been found to be coupled to the oxidation of pyruvate, citrate cycle intermediates (Sacktor, 1954;Gregg et. al .…”
who served as Cochairman of the Supervisory Committee; and, particularly, to Dr. Donald L. Silhacek, who served as Chairman of the Supervisory Comjnittee and under whose direction this study was carried out, and whose encouragement, advice and interest in this study provided a great deal of inspiration and V7ho aided in developing and molding the author's rf^search philosophy.
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