Rattail fescue, a winter annual grass weed, has been increasing in Pacific Northwest (PNW) dryland cereal producing areas. Although rattail fescue is not a new weed species in the PNW, its incidence is expanding rapidly in circumstances where soil disturbances are minimized such as in direct seed systems. Options for effective rattail fescue control in winter wheat cropping systems have not been adequately investigated and need to be developed. Rattail fescue control with herbicide treatments was investigated in imidazolinone-resistant winter wheat using imazamox and other herbicides. Across multiple sites and two growing seasons, crop injury from herbicide treatments was minor to negligible with some exceptions. Treatments containing imazamox or mesosulfuron produced minor, transient winter wheat crop injury at some locations in some years. With the exception of flufenacet applied preemergence (PRE), control of rattail fescue in wheat was variable with single herbicide applications, but improved with sequential herbicide treatments. Rattail fescue biomass was greatly reduced by several treatments especially those containing flufenacet or from sequential herbicide application. Crop yield varied among sites due to growing season precipitation, and in some cases from rattail fescue control or herbicide related crop injury.
Small broomrape is an annual, parasitic weed that was discovered recently in Oregon's red clover seed production system. Field experiments were conducted in 2002 and 2003 at two locations to evaluate 10 herbicide treatments applied after small broomrape emergence in red clover. Bentazon, bromoxynil, glyphosate, imazamox, imazamox plus bentazon, imazethapyr, MCPA, and pendimethalin were evaluated. Small broomrape density, small broomrape seed viability after treatment, and clover injury and seed yield were quantified. Small broomrape control with imazamox, glyphosate, and imazamox plus bentazon treatments was greater than the nontreated check in both years. However, imazamox and imazamox plus bentazon treatments were the only herbicide treatments that consistently exhibited a high level of crop safety, reduced small broomrape density, and did not reduce red clover yield. Herbicide treatments did not prevent production of viable small broomrape seeds. Future research is needed to develop control options that will prevent red clover yield loss and viable small broomrape seed production when applied before small broomrape emergence.
The regulated transport of proteins across the nuclear envelope occurs through nuclear pore complexes (NPCs), which are composed of .30 different protein subunits termed nucleoporins. While some nucleoporins are glycosylated, little about the role of glycosylation in NPC activity is understood. We have identified loss-of-function alleles of ALG12, encoding a mannosyltransferase, as suppressors of a temperature-sensitive mutation in the gene encoding the FXFG-nucleoporin NUP1. We observe that nup1D cells import nucleophilic proteins more efficiently when ALG12 is absent, suggesting that glycosylation may influence nuclear transport. Conditional nup1 and nup82 mutations are partially suppressed by the glycosylation inhibitor tunicamycin, while nic96 and nup116 alleles are hypersensitive to tunicamycin treatment, further implicating glycosylation in NPC function. Because Pom152p is a glycosylated, transmembrane nucleoporin, we examined genetic interactions between pom152 mutants and nup1D. A nup1 deletion is lethal in combination with pom152D, as well as with truncations of the Nterminal and transmembrane regions of Pom152p. However, truncations of the N-glycosylated, lumenal domain of Pom152p and pom152 mutants lacking N-linked glycosylation sites are viable in combination with nup1D, suppress nup1D temperature sensitivity, and partially suppress the nuclear protein import defects associated with the deletion of NUP1. These data provide compelling evidence for a role for glycosylation in influencing NPC function.
A survey of peanut fields for thrips on selected and confirmed hosts of the tomato spotted wilt virus (TSWV) was conducted from November 24, 1986 to April 6, 1987. Berlese separators were utilized to extract thrips from plant samples. The only known thrips vector of TSWV collected during this study was Frankliniella occidentalis. Adult and immature forms of F. occidentalis were common on several weed hosts throughout the winter months. Twelve additional species of thrips were collected during the course of this study.
Annual ryegrass has been proposed as a cover crop in the corn–soybean cropping systems of the U.S. Midwest because of its low seed cost, rapid establishment, contribution to soil quality, weed suppressive abilities, and susceptibility to common broad-spectrum herbicides. However, cover crops can reduce the subsequent main crop yield by creating unfavorable germination and emergence conditions, harboring pests, and if not controlled, competing with the main crop. This study, conducted in Illinois, Oregon, and Tennessee, investigated the efficacy of glyphosate for annual ryegrass winter cover crop removal. Glyphosate at 415, 830, and 1,660 g ae/ha was applied to annual ryegrass at late tiller, second node, boot, and early flowering stages. Annual ryegrass control was consistently maximized with the highest glyphosate rate applied at the boot or early flower stage. Annual ryegrass biomass was generally the lowest with the highest rate of glyphosate applied at the earlier stages. Overall, no single application timing at any glyphosate rate provided complete control or biomass reduction of the annual ryegrass cover crop. A sequential herbicide program or a glyphosate plus a graminicide tank-mix probably will be needed for adequate annual ryegrass stand removal.
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