Plastic petri dishes and microtitration plates were electrically charged by a glow discharge unit installed in a vacuum evaporator. Charged and uncharged plates, as well as plates commercially treated for tissue culture, were inoculated with Vero and BHK-21 cell lines; secondary cultures of monkey kidney, chicken lung, canine kidney, and embryonic bovine kidney; and primary chicken embryo fibroblasts and chicken lung cells. All cell cultures grew normally on petri plates charged with the covers open. Growth rate and cell density compared favorably with growth on the commercial tissue culture plates; cell growth was somewhat less dense, however, on plates charged with the covers closed. Charged plates could be sterilized by ultraviolet light and ethylene oxide with no adverse effects on cell growth. Cells inoculated onto plates charged up to 7 months before inoculation grew as well as on freshly charged plates.

Relative fatty acid compositions of 37 enterococci were examined by gas chromatography. Streptococcus faecalis, S. faecium, and S. faecium var. durans yielded similar fatty acid patterns. Strains of S. faecium var. casseliflavus, and a motile yellow-pigmented streptococcus, contained very low levels of C9:o0 on August 5, 2020 by guest

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The most common viruses found in foods are the enteroviruses, which because of the large number of serotypes and lack of group antigens, require the use of individual antisera to identify each virus. The standardized Lim Benyesh-Melnick enterovirus typing pools, distributed by the NIH, reduce the number of tests required to identify most of the enteroviruses of human origin. The 8 pools are capable of identifying 37 enteroviruses. We have adapted these typing pools for immuno-electron microscopy (IEM), to replace more timeconsuming serological tests.