Differentiation of Some Enterococci by Gas Chromatography

Abstract

Relative fatty acid compositions of 37 enterococci were examined by gas chromatography. Streptococcus faecalis, S. faecium, and S. faecium var. durans yielded similar fatty acid patterns. Strains of S. faecium var. casseliflavus, and a motile yellow-pigmented streptococcus, contained very low levels of C9:o0 on August 5, 2020 by guest

Isolation, identification and antibiogram profile of bacteria isolated from dental caries patients of Mymensingh district of Bangladesh

“…Andrewes, 1906 andElliot et al (1990) were reported the morphological characteristics of Streptococcus mitis are almost similar with the current study. Streptococcus salivarius showed the cultural and morphological characteristics were found closely similar with those that reported by Kathry et al (1984), Facklam et al (1977) and Amstein et al (1973). The cultural and morphological characteristics of Staphylococcus aureus recorded in this study is agreed with those that reported by Comolai et al (2008) and Hanselman et al (2009).…”

Gas-liquid chromatography of the fatty acids of Streptococcus faecalis with a fused silica capillary column

“…In addition, the di-unsaturated 18-carbon acid (18:2) was completely resolved from both oleic (18: 1; 9, 10) and cis-vaccenic (18:1, 11, 12) acids. Thus, in contrast to the results obtained with packed columns in earlier studies [5][6][7][8], use of the capillary column allowed for accurate quantitative determination of all of the fatty acid components of S. faecalis includin~ the positional isomers of unsaturated acids as shown in Table 1. Each of the five strains examined contained palmitic (16:0) and cis-vaccenic (18 : 1; A11) acid as the two major acids with small (1-4%) to moderate (5-13%) amounts of myristic (14:0) stearic (18 : 0), palmitoleic (16 : 1; A9), oleic (18: 1; A9), and lactobacillic (19 cyc; 11,12) acids.…”

“…In recent years, gas-liquid chromatography (GLC), mass spectrometry (MS), and associated analytical techniques have been used to study the chemical composition and metabolic activity of microorganisms as a basis for their classifications [1][2][3][4][5]. Chemical data such as cellular fatty acids have provided valuable information for the recognition of genus or species of various bacteria including streptococci [5][6][7][8].…”

“…In recent years, gas-liquid chromatography (GLC), mass spectrometry (MS), and associated analytical techniques have been used to study the chemical composition and metabolic activity of microorganisms as a basis for their classifications [1][2][3][4][5]. Chemical data such as cellular fatty acids have provided valuable information for the recognition of genus or species of various bacteria including streptococci [5][6][7][8]. In earlier work with streptococci in this laboratory [6] as well as in the extensive studies of Drucker [7,8] and others [5], GLC fatty acid analysis was done with packed columns containing polar or non-polar stationary phase materials.…”

“…Chemical data such as cellular fatty acids have provided valuable information for the recognition of genus or species of various bacteria including streptococci [5][6][7][8]. In earlier work with streptococci in this laboratory [6] as well as in the extensive studies of Drucker [7,8] and others [5], GLC fatty acid analysis was done with packed columns containing polar or non-polar stationary phase materials. With packed columns, not all of the acids of a complex bacterial mixture are separated; however, most can be resolved on a fused silica GLC capillary column [9].…”