Isolation, identification and antibiogram profile of bacteria isolated from dental caries patients of Mymensingh district of Bangladesh

Abstract

This study was carried out for the evaluation of causative agents of dental plaques which leads to dental caries. A total of 76 samples collected from the dental caries patients of various age and sex were processed on different bacterial isolation media like Tryptose Soya Agar, Nutrient Agar, and MacConkey Agar. Total viable count of bacteria was ranged from 0.8×10 5 to 2.9×10 5 . Out of total 184 isolates bacterial species were Streptococcus mutans (28.80%), Streptococcus mitis (23.91%), Staphylococcus aureus (28.26%) and Streptococcus salivarius (19.02%). The biochemical properties of the isolates were tested according to Bergey's Manual of Systematic Bacteriology and UK Standards for Microbiology Investigation. Antibiotic Susceptibility testing of different species of bacteria were performed according to Kirby Bauer disc diffusion method on Muller Hinton Agar by using commercial antibiotic discs. Effect of the bacterial isolates on different age groups was studied where the age group of 26 to 35 years was found to have highest prevalence (32.60%) of infection while the lowest prevalence (10.86%) of infection was seen in the group of below 15 years of age. A relative study was also performed on caries patients according to their economic status and gender where Higher Middle Economic Class was in highest percentage (35.32%) of infection. Different species of bacteria showed various sensitivity patterns to several kinds of antibiotics. Regular use of tooth paste containing triclosan and fluoride may be beneficial for prevention of dental caries instead of using antibiotics presented in this study.

Molecular Characterization and Antibiogram Study of Bacteria Isolated from Dental Plaque Samples from Dental Carries Patients in Northern Bangladesh

“…This study's results corroborate those of [23], which found that the prevalence of dental caries was highest among those aged 31 to 40. Similar to the current study [24], revealed that the maximum prevalence (32.60%) of infection was established in the 26-35 age group, while the lowest prevalence (10.18%)…”

“…It discovered that a more significant proportion of females (80%) were affected by dental caries, which is consistent with the current study's findings. The bacterial frequency based on isolates was more or less similar to the study of [13,24], which identified Almost all of the bacteria in this study are pathogenic, which can cause tooth decay. We noticed that most antibiotics were highly resistant, that's why it is challenging to control dental decay.…”

Prevalence of Methicillin and β−Lactamase Resistant Pathogens Associated with Oral and Periodontal Disease of Children in Mymensingh, Bangladesh

“…Various species of the genus Streptococcus, Staphylococcus, Lactobacillus, Enterococcus, Corynebacterium, Veillonella and Bacteroids are predominant bacteria in the human oral cavity. Mutans group Streptococci (Streptococcus mutans and Streptococcus sobrinus), mittis group Streptococci (Streptococcus sanguinis, Streptococcus oralis, and Streptococcus gordonii) and Salivarius group (Streptococcus salivarius) are major constituents of human oral flora [8][9][10][11]. High levels of S. mutans and early colonization are considered key risk factors for the development of dental caries in both children and adults [12,13].…”

Isolation, characterization and antibiogram studies of bacteria isolated from ready-to-eat foods sold at different places of Dinajpur district, Bangladesh

“…This indicates the need to determine the microbial load or status of RTE foods to prevent foodborne diseases and promote health and well-being. In Bangladesh, some previous microbiological studies conducted to know respiratory bacterial agents from buffalo (Akter et al, 2018), bacterial load in dental caries (Borty et al, 2015), bacterial load of poultry meat (Hossain et al, 2015), bacteria from mobile phones of student (Hussein et al, 2020), bacteria isolated from table eggs (Islam et al, 2018), bacterial species isolated from milk (Munsi et al, 2016), bacterial pathogens from egg shell, egg yolk, feed and air samples (Parveen et al, 2017), bacterial loads in butter and cheese (Parvin et al, 2016), bacterial population of raw milk (Rana et al, 2021) and antibiotic sensitivity of bacteria in raw milk (Talukder and Ahmed 2016). A very few studies are conducted to assess the bacterial load and their antibiogram in RTE foods, however, this study is the first study from the current study location for assessing the bacterial load and their antibiogram in RTE foods.…”