No abstract

Summary β‐1,6‐glucan is an important cell wall component of Candida albicans. Deleted mutants of the two β‐1,6‐glucan synthase genes KRE6 and SKN1 are viable albeit with a range of defects including slow growth. It remains unclear whether β‐1,6‐glucan synthesis is not required under culture conditions or compensatory mechanisms exist in C. albicans. Here, we report that depleting β‐1,6‐glucan synthases leads to a significant increase in cell wall chitin levels through the posttranscriptional regulation of the chitin synthase Chs3 which maintains cell viability. And depleting β‐1,6‐glucan synthases in chs3Δ/Δ cells results in cell death. The elevation of cell wall chitin is mediated by the activation of the PKC signaling pathway and an unknown pathway(s) involving Ca2+–calcineurin. Also, kre6Δ/Δ skn1Δ/Δ cells are not more susceptible to caspofungin, the antifungal drug that inhibits β‐1,3‐glucan synthases, suggesting that β‐1,3‐glucan has no role in compensating β‐1,6‐glucan synthesis. Given the vital importance of elevating chitin synthesis in the absence of β‐1,6‐glucan synthesis in C. albicans, antifungal drugs targeting β‐1,6‐glucan and chitin synthesis could be used in combination therapies.

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The Once‐in‐a‐Century extreme rainstorm event caused severe floods over Henan province during July 18–21, 2021, which resulted in large casualty and property losses. Although the rainstorm event occurred in Henan after July 18, the excessive rainfall had occurred to the east of Henan before July 18, with the 4‐day accumulated rainfall exceeding +130 mm during July 14–17, 2021. How the rainfall evolving westward and intensifying after July 18 remained a puzzle, which is the focus of this study. The prerainstorm stage (July 14–17) was related to the South Asian High (SAH) extending eastward and the western Pacific subtropical high (WPSH) extending northwestward, and a low vortex between the SAH and WPSH caused above‐normal rainfall to the east of Henan. The rainstorm stage (July 18–21) was associated with an inverted trough and excessive southerly and southeasterly water vapor transportation above Henan, which resulted from the combined effects of a deep trough in the upper troposphere and typhoon activities. Additionally, three subseasonal forecasting systems predicted this rainstorm event 3 days in advance, with the European Center for Medium Range Weather Forecasts (ECMWF) performing the best, which was related to a better prediction of the inverted trough and the water vapor transportation in the middle‐lower troposphere. These results advance our understanding of the extreme rainstorm event in July 2021 in Henan.

PP2A-like phosphatases share high homology with PP2A enzymes and are composed of a catalytic subunit and a regulatory subunit. In Candida albicans, the PP2A-like catalytic subunit SIT4 regulates cell growth, morphogenesis, and virulence. However, the functions of its regulatory subunits remain unclear. Here, by homology analysis and co-IP experiments, we identified two regulatory subunits of SIT4 in C. albicans, SAP155 (orf19.642) and SAP190 (orf19.5160). We constructed sit4 / , sap155 / , sap190 / , and sap155 / sap190 / mutants and found that deleting SAP155 had no apparent phenotypic consequence, while deleting SAP190 caused slow growth, hypersensitivity to cell wall stress, abnormal morphogenesis in response to serum or genotoxic stress (HU and MMS), less damage to macrophages, and attenuated virulence in mice. However, deleting both SAP155 and SAP190 caused significantly stronger defects, which was similar to deleting SIT4. Together, our results suggest that SAP190 is required for the function of SIT4 and that SAP155 can partially compensate for the loss of SAP190 in C. albicans. Given the vital role of these regulatory subunits of SIT4 in C. albicans physiology and virulence, they could serve as potential antifungal targets.