Three genes, mcbABC, that drive the biosynthesis of marinacarbolines, have been elucidated through genome mining, gene inactivation, heterologous expression, feeding, and site-directed mutagenesis experiments. McbB is highlighted as a novel enzyme for the β-carboline core construction, which involves a Pictet-Spengler cyclization process and requiring E97 for biochemical activity.
Drei Gene, mcbABC, die für die Biosynthese von Marinacarbolinen zuständig sind, wurden mithilfe von Genom‐Mining, Gen‐Inaktivierung, heterologer Expression, Befütterung und ortsgerichteter Mutagenese aufgeklärt. McbB ist ein neuartiges Enzym für den Aufbau des β‐Carbolinkerns unter Beteiligung einer Pictet‐Spengler‐Cyclisierung; dabei wird E97 für biochemische Aktivität benötigt.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.