The micro-morphological examinations of the leaf lamina, petiole and stem for Camellia sinensis (L.) Kuntze (Theaceae) was carried out using a variety of microscopic techniques widely implemented in the area of medicine. The objective of this study was the micromorphological characterization of stem, petiole, lamina, stomata, leaf trichomes and other internal characters. The anatomical studies included the cross section of stem and leaf of Camellia sinensis thereby exhibiting a typical pattern of arrangement of tissues similar to woody plants. Some idioblastic sclereids like astrosclereids, osteosclereids were found in the medullary parenchyma of tea stem and leaf. Large numbers of sclereids were found mainly in the parenchymatous tissue of leaf petiole. Other micro-morphological features like trichomes, stomata, and different tissue layer were also recorded. The leaf trichomes were unicellular, long and densely present in the lower surface of immature leaf but a decrease in amount of trichomes was seen in the mature leaf making it a prime taxonomic feature of the tea leaf. The microscopic morphological analysis of the stem, petiole, lamina, stomata, leaf trichomes of Camellia sinensis can be used for its identification. In addition, these techniques can be further implemented for the taxonomic characterization thereby establishing a genetic relationship and solving taxonomic disputes in the field of plant systematics.
Nanotechnology is the integrative science in the field of physics, chemistry and biology. For the synthesis of silver nanoparticles, a simple approach was applied using
Firmiana
colorata
(Roxb.) aqueous leaf extract. During the synthesis of this silver nanoparticle, the solution color changes from green to deep brown due to the reduction of silver. The phytocompounds present in the
Firmiana
colorata
(Roxb.) leaf extract acts as a reducing as well as a capping agent. Identifying the presence of bioactive compounds responsible for the reduction of silver was extensively characterized by UV–Vis spectrophotometer, FTIR, SEM, and EDX. Moreover, to know the efficacy of the silver nanoparticles (AgNps) antioxidant and antimicrobial studies were evaluated against the human pathogenic bacteria. Furthermore, GC–MS analysis of the leaf extract of
Firmiana
colorata
has been done followed by the
in-silico
molecular docking against the Anti-inflammatory and oxidative protein. Here within this study, a comparative evaluation was done among the
Firmiana
colorata
(Roxb.) leaf extract and the synthesized silver nanoparticles. Results indicate that ethnomedicinally lesser known
Firmiana
colorata
(Roxb.) and AgNps have the potency to act as anti-inflammatory, antioxidative, and antimicrobial agents.
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