Genetic improvement of livestock productivity can be done through molecular selection on the genes controlling growth traits. Genetic polymorphism of the growth family (GH, GHRH, and PIT1) genes were studied in local swamp buffalo (106 hds.) from a government buffalo breeding station (46 heads) and smallholders (60 heads) in North Tapanuli District, North Sumatra Province. Genotype variants of the three genes were identified by PCR-RFLP method using restriction enzymes of MspI (GH gene), HaeIII (GHRH gene) and HinfI (PIT -1 gene). Genotyping on individual GH_g.1547T>C, GHRH_g.4666G>C, and PIT -1_g.1256G >A loci resulted only one type genotype, respectively TT, CC, an d AA, with one type of allele, respectively T, C, and A. Heterozygosity observation (Ho) and expectation (He) values values and the PIC value for each locus was 0.00. It could be suggested to increase genotype frequenciest of the three growth genes that are positively associated with the growth traits and economic traits of the buffalo.
The molecular selection of the genes influencing growth qualities can be used to genetically improve the production of native swamp buffaloes. Local swamp buffalo (60 hds.) maintained by smallholders in Pandeglang Regency, Banten Province, were examined for the genetic polymorphism of the growing family (GH, GHRH, and PIT1) genes. By using restriction enzymes of MspI (GH gene), HaeIII (GHRH gene), and HinfI (PIT-1 gene), the genotyping of GH g.1547T>C, GHRH g.4666G>C, and PIT-1g.1256G>A SNPs was found by polymerase chain reaction-restriction fragment polymorphism (PCR-RFLP). Genotyping on the respective GH_g.1547T>C, GHRH_g.4666G>C, and PIT-1_g.1256G>A loci, however, resulted in only one type of genotype i.e. CC, GG, and AA with one type of allele i.e. C, G, and A successively. Therefore, the frequencies were 100% or monomorphic for those genotypes and alleles of each growth gene. Also, the PIC values of each locus were 0.00, as were the heterozygosity observation (Ho) and expectation (He) values. It is possible to suggest increasing the genotype frequency of the SNPs of the relevant growth genes that are favorably correlated with the growth traits and associated economic traits of the observed local swamp buffalo in this area.
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