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For the first time, nickel hydroxide nanoparticles (Ni(OH) 2 NPs) grown on 316L stainless steel foam were used as a non-enzymatic electrochemical glucose sensor. The Ni(OH) 2 /SSF-316L was elaborated by applying a simple ultrafast CV method without nickel salts addition. Ni(OH) 2 /SSF-316L was characterized by SEM and XRD. The electrochemical behavior was investigated by CV, EIS, and amperometric measurements. The fabricated sensor revealed higher sensitivity 1062 μA mM À 1 cm À 2 , wide linear range from 1.0 μM to 4.0 mM with a low detection limit of 2.0 μM and good selectivity. In addition, real sample analysis was performed for controlled glucose in real blood serum.