The aim of this study was to verify the effect of the energy source for a short‐term diet supplementation on follicular dynamics, ovarian response and oocyte recovery in goats. Thirty Anglo Nubian crossbred does received a diet for 4 weeks to satisfy the nutritional requirements of breeding for adult non‐dairy goats. Seven days prior to oocyte recovery (OR), a group of does (n = 10) was supplemented with ground full‐fat linseed in the diet (Diet A), whereas a second group of does (n = 10) received crude glycerine in the diet (Diet B). The total mixed ration (TMR) diet was maintained as the Control Diet (n = 10). All animals were oestrous‐synchronized by the use of a progesterone insert for 12 days prior to OR. Follicles were stimulated by using pFSH (five 40‐mg/ml doses) during the supplementation time. At OR, follicles were counted and recovered oocytes were classified as viable or degenerated. Follicular dynamics was monitored by ultrasonography, and plasma glucose, cholesterol and triglyceride levels were measured during supplementation. Glucose was higher in Diet B and cholesterol in Diet A. Diet B had a lower proportion of small (<3 mm) and large follicles (≥3 mm; p = 0.01). The follicular growth rate was higher in Diet A (p < 0.01), with follicles emerging in the 5th day of supplementation. No differences were observed for follicles counted and oocytes recovered. Thus, the type of energy source supplemented for a short term was capable to alter the follicular dynamics, without affecting the proportion of morphologically viable oocytes upon recovery.
This study evaluated the effect of increasing the nutritional plane in finishing diets on meat quality traits of culled adult goats. Forty-one crossbred does were divided into four groups: baseline diet formulated to meet 100% of the nutritional requirements (BD); BD + 30% (BD30); + 60% (BD60); + 90% (BD90) of the nutritional level of BD. The goats received the experimental diets for 28 days. The protein profile of the longissimus lumborum muscle was evaluated using 1D SDS-PAGE and mass spectrometry. Cholesterol and albumin concentrations were highest in the BD group. There were no alterations in loin proximate composition or tissue composition. However, there was a significant reduction in the PUFA content in groups BD60 and BD90 compared with the group fed the baseline diet. Of 37 protein bands identified, 35 showed lower intensities in group BD90 than in BD and 22 bands showed lesser expression in groups BD60 and BD90 than in BD. The multivariate model identified 14 band proteins involved in group-diet segregation. Therefore, increasing the nutrient supply for finishing goats did not have marked positive effects on carcass. A lower expression of these proteins can have a profound impact on the qualitative aspects of the meat product.
This study evaluated the impact of nutritional status of Morada Nova sheep at lambing on the reproductive and productive performance and on the survival of lambs in early weaning system. Nineteen, Morada Nova sheep were assigned to two groups according to body condition score (BCS) at lambing: low BCS (n = 11) and high BCS (n=8) with body condition respectively of (mean ± SD) 2.0 ± 0.3 e 2.9 ± 0.1. From birth until lamb weaning (45 days), sheep were weighed weekly and checked the BCS, loin subcutaneous fat thickness, loin depth, hematological profile, milk composition and production, and every three days, we measured the uterine diameter. Lamb weightings were performed up to one week after weaning (52 days). The lower availability of muscle and fat reserves in the low BCS group negatively affected milk production and consequently performance of suckling lambs. However, the results indicated that the uterine involution process, the reproductive parameters including prolificacy, rate of multiple births, number of white blood cells, milk quality, body weight of lambs at birth and mortality rates were not affected by the body condition. The results allowed to describe the responsiveness to opposite nutritional status of Morada Nova sheep, showing their characteristics of adaptation. Key words: Body condition. Ewes. Milk. Lamb. Uterine involution. ResumoO objetivo do trabalho foi verificar o impacto do estado nutricional de ovelhas Morada Nova ao parto sobre o seu desempenho reprodutivo e produtivo e sobre a sobrevivência de cordeiros em sistema de desmame precoce. Foram utilizadas 19 ovelhas Morada Nova, divididas em dois grupos de acordo com o escore da condição corporal ECC ao parto: grupo ECC baixo (n = 11) e ECC alto (n = 8) com escore médio (± DP) respectivamente de 2.0 ± 0.3 e 2.9 ± 0.1. A partir do parto até o desmame dos cordeiros (45 dias), as mães foram pesadas semanalmente e verificado o ECC, espessura da gordura subcutânea lombar, profundidade do lombo, perfil hematológico, composição e produção leiteira e a cada três dias
This study was aimed to investigate the follicular growth of Morada Nova sheep supplemented with crude glycerin before mating. Eighteen ewes were subjected to follicular wave and estrus synchronization with three injections of D-cloprostenol administered via IM, on days -14, -7 and -1 before mating. Animals were randomized into two groups, the control (n = 9), which received an oral drench of solution of 200 mL of saline solution, and the glycerin group (n = 9), which was supplied with 200 mL glycerol (n = 9), administered as an oral drench of crude glycerin in saline solution (9:1), 1h after morning feeding. Glycerin was supplied for 6 days, beginning 7 days from the mate. Growth follicular pattern was monitored daily by transrectal ultrasonography during the period of glycerin supplementation. Blood samples were taken during glycerin administration for progesterone and glucose analysis. There was a significant increase (P < 0.001) in blood glucose after supplementation with glycerin (mean of 66.2 ± 1.3 vs 56.5 ± 0.8 mg/dL). The greater availability of energy near to ovulation was beneficial to Morada Nova ewes, leading to a significant increase (P < 0.001) of large follicles (> 3 mm). At diagnosis of pregnancy, it was observed that approximately 87% (13/15) (87.5% control group vs. 85.7% glycerin group) of the ewes were pregnant. The group treated with glycerin presented an increase of more than 40% of prolificacy (1.7 ± 0.3 vs. 1.2 ± 0.2; P=0.21). In conclusion, the monitoring of follicular growth reveals that crude glycerin increases the follicular population of Morada Nova ewes, when applied before mating.
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