No abstract

It is well known that the level of anesthesia obtained by intraperitoneal injection is variable and may alter cardiac function. In this study, we compared the effects of different anesthetics on cardiac function with the conscious state using high-resolution two-dimensional echocardiography in nonischemic and ischemic mice. Eighty-four mice were tested before and after surgery with ligation of the coronary artery. All 84 mice were studied in the conscious state and under high-dose intraperitoneal anesthesia. Twenty-two of 84 mice were studied under low-dose intraperitoneal anesthesia. Another 22 mice were also studied under gas anesthesia and spontaneous breathing. Experiments in the conscious state were performed by two investigators before the administration of anesthesia: one investigator held the animal and the transducer and the other operated the ultrasound equipment. Left ventricular systolic function was measured, and measurements obtained after surgery were compared with infarcted areas assessed by histological staining. Results showed that both high- and low-dose intraperitoneal anesthesia significantly reduced heart rates and left ventricular contractility in both pre- and postsurgical mice as opposed to conscious mice (P < 0.01). There were significantly higher correlation coefficients between mean fractional area change (FAC) and infarcted area in conscious state compared with high-dose intraperitoneal anesthesia (P < 0.05). The correlation coefficient between FAC and infarcted area during gas anesthesia was also significantly higher compared with high-dose intraperitoneal anesthesia (P < 0.05). In conclusion, conscious experiments or the use of gas anesthesia is preferred for echocardiographic assessment of cardiac function in mice because intraperitoneal injection significantly induces a significant reduction in heart rate and left ventricular systolic function.

Cardiovascular stress testing plays a crucial role in the initial detection of coronary artery disease. In exercise stress echocardiography, the rapid acquisition of echocardiographic images is critical for accuracy. Real-time three-dimensional echocardiography permits the rapid acquisition of a volumetric data set that includes the entire left ventricle and allows the review of multiple, standard two-dimensional images from a single volumetric data set. Volumetric data can be obtained using both apical and parasternal windows. Often, satisfactory images are obtained in the majority of both prestress and poststress imaging using only an apical volume set. The following is a review of the current applications of real-time three-dimensional echocardiography in stress testing.

Murine models of cardiac disease are becoming an important tool for studying pathophysiological processes. Development of methods to accurately assess ventricular function are therefore important. The purpose of this study was to evaluate the feasibility of echocardiographic assessment of segmental wall motion abnormalities in a murine model of myocardial infarction. Two-dimensional contrast (C+) and noncontrast (C-) echocardiography were performed in 76 awake mice 2 days before and 2 days after left coronary ligation. The short-axis images obtained with two-dimensional echocardiography and corresponding postmortem cross-sectional histological samples stained with Evans blue dye were each divided into 16 segments, and all matched segments were examined for correlation between wall motion abnormalities and myocardial hypoperfusion. With the use of contrast enhancement, the number of visualized segments was significantly increased (base: C- 86%, C+ 98%; midpapillary: C- 57%, C+ 89%; apex: C- 30%, C+ 74%). Agreement between echocardiographically assessed regional wall motion abnormalities and pathologically determined hypoperfusion in basal, midpapillary, and apical levels were 90%, 93%, and 93%, respectively. Agreement between echocardiographically normal wall motion and pathologically normal findings in basal, midpapillary, and apical levels were 99%, 88%, and 71%, respectively. Thus echocardiographic assessment of segmental wall motion in awake mice was feasible and the accuracy was improved with the use of a contrast agent.

eft coronary artery ligation is one of the most common methods of creating a model of myocardial infarction (MI) in the murine heart and M-mode echocardiography has been used to assess cardiac function because of its higher sampling frequency, which is particularly useful when assessing a heart rate (HR) of 600 beats/min or greater. 1-9 However, the estimation of global left ventricular (LV) function using M-mode echocardiography has limitations when it is applied to an infarcted heart 10 and 2-dimensional (2-D) echocardiography is more suitable. In murine hearts with their small size and high heart rate, however, even this method has been limited in its application for assessment of cardiac function because of the difficulty in obtaining adequate 2-D images.The recent improvements in 2-D echocardiography of a higher resolution and higher frame rate using a highfrequency transducer provide more accessible images for