Although prostate cancer is one of the most common cancers in the male population, its basic biological function at a cellular level remains to be fully understood. This lack of in depth understanding of its physiology significantly hinders the development of new, targeted and more effective treatment strategies. Whilst electrophysiological studies can provide in depth analysis, the possibility of recording electrical activity in large populations of non-neuronal cells remains a significant challenge, even harder to address in the picoAmpere-range, which is typical of cellular level electrical activities. In this paper, we present the measurement and characterization of electrical activity of populations of prostate cancer cells PC-3, demonstrating for the first time a meaningful electrical pattern. The low noise system used comprises a multi-electrode array (MEA) with circular gold electrodes on silicon oxide substrates. The extracellular capacitive currents present two standard patterns: an asynchronous sporadic pattern and a synchronous quasi-periodic biphasic spike pattern. An amplitude of ±150 pA, a width between 50–300 ms and an inter-spike interval around 0.5 Hz characterize the quasi-periodic spikes. Our experiments using treatment of cells with Gd3⁺, known as an inhibitor for the Ca2⁺ exchanges, suggest that the quasi-periodic signals originate from Ca2⁺ channels. After adding the Gd3⁺ to a population of living PC-3 cells, their electrical activity considerably decreased; once the culture was washed, thus eliminating the Gd3⁺ containing medium and addition of fresh cellular growth medium, the PC-3 cells recovered their normal electrical activity. Cellular viability plots have been carried out, demonstrating that the PC-3 cells remain viable after the use of Gd3⁺, on the timescale of this experiment. Hence, this experimental work suggests that Ca2⁺ is significantly affecting the electrophysiological communication pattern among PC-3 cell populations. Our measuring platform opens up new avenues for real time and highly sensitive investigations of prostate cancer signalling pathways.
A novel fabrication process called BETTS (bonding, UV exposing and transferring technique in SU-8) is presented in this paper. SU-8 layers can be transferred and patterned over SU-8 microstructures by means of a removable, flexible and transparent substrate. This substrate is composed of a thin acetate film, which can be also used as a mask, and a cured PDMS layer deposited over it. SU-8 is then spin coated and transferred to the SU-8 structures performing simultaneously the steps of bonding and transferring by UV exposure. Due to the low adhesion between PDMS and SU-8, acetate film removal can be easily performed. BETTS provides easy, irreversible and robust SU-8 to SU-8 bonding, where the absence of oxygen plasma equipment or vacuum systems decreases drastically the fabrication cost and time involved. The reported fabrication process makes it possible to fabricate complex SU-8 three-dimensional structures using a simple and inexpensive procedure and also ensures its compatibility and integration with microfluidic and PCB-MEMS devices. Some specific applications such as multilevel microchannel network, patterned membranes, microchambers and microvalves are reported to demonstrate the potential of the proposed process.
Use of advanced communication technologies, highly integrated control, and programming platforms drastically increases the performance of industrial control systems. That is the case of Motronic, where the synergistic collaboration between industry and academia has led to an advanced distributed network control system. To be commercially successful, it needs to have a low cost and to be robust, even if this requirement implies that it is a custom design and not based on previously existing commercial solutions. Use of standards and off-the-shelf products lower development costs, but usually raise production costs. In this paper, we show that, in certain applications, design of a new system from scratch is more advantageous. This system comprises a set of dynamically reconfigurable local controller nodes, a graphical programming environment, a remote supervision and control system, and a fault-tolerant fiber optical network. TCP/IP connectivity is provided by the use of a local gateway. Motronic is currently being applied in the integrated control of large production plants and in energy and power management industries.
In this paper, a single-use and unidirectional microvalve with low consumption of energy for PCB-based microfluidic platforms is reported. Its activation is easy because it works as a fuse. The fabrication process of the device is based on PCB technology and a typical SU-8 process, using the PCB as a substrate and SU-8 for the microfluidic channels and chambers. The microvalve is intended to be used to impulse small volumes of fluids and it has been designed to be highly integrable in PCB-based microfluidic platforms. The proposed device has been fabricated, integrated and tested in a general purpose microfluidic circuit, resulting in a low activation time, of about 100 μs, and a low consumption of energy, with a maximum of 27 mJ. These results show a significant improvement because the energy consumption is about 84% lower and the time response is about four orders of magnitude shorter if compared with similar microvalves for impulsion of fluids on PCB-based platforms.
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