No abstract

Nitric oxide (NO) is considered a key regulator of plant developmental processes and defense, although the mechanism and direct targets of NO action remain largely unknown. We used phenotypic, cellular, and genetic analyses in Arabidopsis thaliana to explore the role of NO in regulating primary root growth and auxin transport. Treatment with the NO donors S-nitroso-N-acetylpenicillamine, sodium nitroprusside, and S-nitrosoglutathione reduces cell division, affecting the distribution of mitotic cells and meristem size by reducing cell size and number compared with NO depletion by 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (cPTIO). Interestingly, genetic backgrounds in which the endogenous NO levels are enhanced [chlorophyll a/b binding protein underexpressed 1/NO overproducer 1 (cue1/nox1) mirror this response, together with an increased cell differentiation phenotype. Because of the importance of auxin distribution in regulating primary root growth, we analyzed auxin-dependent response after altering NO levels. Both elevated NO supply and the NO-overproducing Arabidopsis mutant cue1/nox1 exhibit reduced expression of the auxin reporter markers DR5pro:GUS/GFP. These effects were accompanied by a reduction in auxin transport in primary roots. NO application and the cue1/nox1 mutation caused decreased PIN-FORMED 1 (PIN1)-GFP fluorescence in a proteasome-independent manner. Remarkably, the cue1/nox1-mutant root phenotypes resemble those of pin1 mutants. The use of both chemical treatments and mutants with altered NO levels demonstrates that high levels of NO reduce auxin transport and response by a PIN1-dependent mechanism, and root meristem activity is reduced concomitantly.cell division and elongation | plant growth regulator | root development N itric oxide (NO) is a signaling molecule involved in a variety of physiological processes during plant growth and development and also is an important modulator of disease resistance. Extensive research has shown that NO is involved in the promotion of seed germination, photomorphogenesis, mitochondrial activity, leaf expansion, root growth, stomatal closure, fruit maturation, senescence, and iron metabolism (as reviewed in ref. 1). NO also is important for defense response, playing key roles in the activation of defense genes (e.g., pathogenesis-related protein 1), in phytoalexin production, and in modulation of programmed cell death (1-3). The mechanism for NO signal transduction, plant resistance to pathogens and cell death, cellular transport, basic metabolism, and photosynthesis frequently occurs through an NO-induced change in transcription (4).Additionally, NO is produced in plant tissues by two major pathways, one enzymatic and the other nonenzymatic (5). The enzymatic pathway of NO production is being studied thoroughly, and much information about the type and subcellular localization of the enzymes involved is available. Different enzymes have been identified that catalyze the synthesis of NO from two different substrates, nitrate and argi...

Lipoxygenases are ubiquitous enzymes in eukaryotes. In plants, lipoxygenases are involved in the synthesis of the hormone jasmonic acid that regulates plant responses to wounding and, in addition, is an inducer of tuberization in potato. We have isolated potato lipoxygenase cDNA clones. From their deduced amino acid sequences, three distinct classes are defined (Lox1, Lox2, and Lox3). They are encoded in gene families that display organ-specific expression, lox1 being expressed mostly in tubers and roots, lox2 in leaves, and lox3 in leaves and roots. Consistent with their organ-specific expression pattern, Lox1 expressed in bacteria preferentially uses as substrate linoleic acid, abundant in membrane lipids of tubers, whereas linolenic acid, prevalent in leaves, is the preferred substrate for the other two classes of lipoxygenase. Analyses on reaction products of the enzymes expressed in bacteria reveal that Lox1 primarily produces 9- hydroperoxides. In contrast, the jasmonic acid precursor, 13-hydroperoxylinolenic acid, is the major product of the action of Lox2 and Lox3 on linolenic acid. Upon wounding, the levels of Lox2 and Lox3 transcripts rise markedly in leaves. While Lox3 mRNA accumulation peaks as early as 30 min after wounding, Lox2 shows a steady increase over a 24-h time course, suggesting different roles for these lipoxygenase isoforms in the synthesis of the plant hormone jasmonic acid.

Hydroperoxide lyases (HPLs) catalyze the cleavage of fatty acid hydroperoxides to aldehydes and oxoacids. These volatile aldehydes play a major role in forming the aroma of many plant fruits and flowers. In addition, they have antimicrobial activity in vitro and thus are thought to be involved in the plant defense response against pest and pathogen attack. An HPL activity present in potato leaves has been characterized and shown to cleave specifically 13-hydroperoxides of both linoleic and linolenic acids to yield hexanal and 3-hexenal, respectively, and 12-oxo-dodecenoic acid. A cDNA encoding this HPL has been isolated and used to monitor gene expression in healthy and mechanically damaged potato plants. HPL gene expression is subject to developmental control, being high in young leaves and attenuated in older ones, and it is induced weakly by wounding. HPL enzymatic activity, nevertheless, remains constant in leaves of different ages and also after wounding, suggesting that posttranscriptional mechanisms may regulate its activity levels. Antisense-mediated HPL depletion in transgenic potato plants has identified this enzyme as a major route of 13-fatty acid hydroperoxide degradation in the leaves. Although these transgenic plants have highly reduced levels of both hexanal and 3-hexenal, they show no phenotypic differences compared with wild-type ones, particularly in regard to the expression of wound-induced genes. However, aphids feeding on the HPL-depleted plants display approximately a two-fold increase in fecundity above those feeding on nontransformed plants, consistent with the hypothesis that HPL-derived products have a negative impact on aphid performance. Thus, HPL-catalyzed production of C6 aldehydes may be a key step of a built-in resistance mechanism of plants against some sucking insect pests.

No abstract

During the past two decades, nitric oxide (NO) has evolved from a mere gaseous free radical to become a new messenger in plant biology with an important role in a plethora of physiological processes. This molecule is involved in the regulation of plant growth and development, pathogen defence and abiotic stress responses, and in most cases this is achieved through its interaction with phytohormones. Understanding the role of plant growth regulators is essential to elucidate how plants activate the appropriate set of responses to a particular developmental stage or a particular stress. The first task to achieve this goal is the identification of molecular targets, especially those involved in the regulation of the crosstalk. The nature of NO targets in these growth and development processes and stress responses remains poorly described. Currently, the molecular mechanisms underlying the effects of NO in these processes and their interaction with other plant hormones are beginning to unravel. In this review, we made a compilation of the described interactions between NO and phytohormones during early plant developmental processes (i.e. seed dormancy and germination, hypocotyl elongation and root development).

The effect of ozone treatment on the postharvest quality of strawberry was evaluated. Strawberry fruits (Fragaria x ananassa Duch. cv. Camarosa) were stored at 2 degrees C in an atmosphere containing ozone (0.35 ppm). After 3 days at 2 degrees C, fruits were moved to 20 degrees C to mimic retail conditions (shelf life). The changes in several quality parameters such as fungal decay, color, sugar and acids distribution, and aroma were evaluated during the strawberries' shelf life. Ozone treatment was ineffective in preventing fungal decay in strawberries after 4 days at 20 degrees C. Significant differences in sugars and ascorbic acid content were found in ozone-treated strawberries. At the end of cold storage, the vitamin C content of ozonated strawberries was 3 times that of control fruits. A detrimental effect of ozone treatment on strawberry aroma was observed, with a 40% reduced emission of volatile esters in ozonated fruits.

An analytical procedure to determine major sugars and organic acids, including vitamin C, in fruits was developed using a C18 Sep-Pak cleanup process and an ion exclusion HPLC column. Dual UV monitoring and refractive index were performed for detection. To attain optimal separation and quantitation, 0.0085N H2SO4 was used as the mobile phase and the column temperature was maintained at 23 °C. This procedure was compared to others for the individual quantitation of sugars, organic acids, and vitamin C. Recovery and reproducibility of this analytical procedure were quite acceptable for strawberry and four other common fruits, allowing the analysis of all the components using a single-injection HPLC analysis in <22 min. Keywords: Simultaneous analysis; high-performance liquid chromatography; sugars; organic acids; vitamin C; strawberry; fruits