1997
DOI: 10.1021/jf9701704
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Rapid Determination of Sugars, Nonvolatile Acids, and Ascorbic Acid in Strawberry and Other Fruits

Abstract: An analytical procedure to determine major sugars and organic acids, including vitamin C, in fruits was developed using a C18 Sep-Pak cleanup process and an ion exclusion HPLC column. Dual UV monitoring and refractive index were performed for detection. To attain optimal separation and quantitation, 0.0085N H2SO4 was used as the mobile phase and the column temperature was maintained at 23 °C. This procedure was compared to others for the individual quantitation of sugars, organic acids, and vitamin C. Recovery… Show more

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Cited by 159 publications
(115 citation statements)
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References 31 publications
(37 reference statements)
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“…In addition, pH, tartaric, malic and citric acid contents for both fruits were established as 4.39 and 4.59; 0.30 mg/100 g and 0.29 mg/100 g; 0.30 mg/100 g and 0.17 mg/100 g and 1104.85 mg/100 g and 732.69 mg/ 100 g, respectively. The amounts of citric, malic and ascorbic acids were reported as 3.21, 1.11 and 0.19 g/kg of FW in strawberry fruits by Perez et al (1997). Tartaric, malic and citric acid contents of some apple cultivars ranged from 0.014 to 0.030 mg/L, 2.73 to 7.07 mg/L and 0.044 to 0.093 mg/L, respectively (Wu et al 2007).…”
Section: Biochemical Properties and Mineral Contentsmentioning
confidence: 99%
“…In addition, pH, tartaric, malic and citric acid contents for both fruits were established as 4.39 and 4.59; 0.30 mg/100 g and 0.29 mg/100 g; 0.30 mg/100 g and 0.17 mg/100 g and 1104.85 mg/100 g and 732.69 mg/ 100 g, respectively. The amounts of citric, malic and ascorbic acids were reported as 3.21, 1.11 and 0.19 g/kg of FW in strawberry fruits by Perez et al (1997). Tartaric, malic and citric acid contents of some apple cultivars ranged from 0.014 to 0.030 mg/L, 2.73 to 7.07 mg/L and 0.044 to 0.093 mg/L, respectively (Wu et al 2007).…”
Section: Biochemical Properties and Mineral Contentsmentioning
confidence: 99%
“…Pear slices were analyzed to determine the amount of ascorbic acid, calcium and cysteine present after storage at 0°C in air for 0, 3, 7 or 10 days. Ascorbic acid was determined by HPLC using the method of Perez et al (1997). Cysteine concentrations were determined by the method of Buwalda et al (1988) and modified by Pesis et al (1998).…”
Section: Residual Cysteine Ascorbic Acid and Calcium Analysismentioning
confidence: 99%
“…Almost all of this production comes from small family farms of 0.05-0.5 hectares. Strawberries are unique with highly desirable taste, flavor, and excellent dietary sources of ascorbic acid, potassium, fibre and other secondary metabolites and also simple sugar sources of energy (Perez et al, 1997;Wang & Galletta, 2002).…”
Section: Introductionmentioning
confidence: 99%