Aedes cells infected with SFV fuse when exposed to pH 6. We have previously shown that 1) this process is a 'fusion from within', 2) a conformational change occurs at the plasma membrane, presumably of the viral spike proteins and 3) the new conformation is stable. The conformation of proteins may be stabilized by disulfide bonds. To examine this, sodium thiosulfate and sodium sulfite at 1 mM concentrations were added to the cells 16 h after infection. Application before lowering the pH had no effect on the fusion. However, application after lowering the pH completely inhibits fusion. This result substantiated that the known conformational change on the cell surface leads to exposition of disulfide bonds which are essential for maintaining the fusogenic conformation. Incorporating the results obtained with dithiothreitol and tetrathionate we conclude that at least three cysteines are involved in fusion. In vitro protein synthesis by cells infected with different types of bovine herpesvirus 1Based on present knowledge strains of bovine herpesvirus l (BHV-1) can be assigned to one out of three major virus types, i.e. BHV-1/type 1, BHV-1/type 2 (with subtypes 2a and 2b), and BHV-1/type 3 (with subtypes 3a and 3b). Comparison of the various virus types and subtypes revealed that BHV-1/subtype 3b viruses were consistently difficult to grow to satisfactory infectivity titers. This was paralleled by delayed appearance of virus-induced cytopathology, concomitant with the production of smaller plaques. The present study shows that the protein synthesis rate at various times after infection (as determined my measuring incorporation of (3SS)methionine into translational products) was more efficiently reduced by subtype 3b strains, when compared with the other viruses. This reduction was followed by a low virus-induced protein synthesis rate. The results imply that some nonimpaired host cell functions could be of importance in determining the efficiency of viral growth. Bovine herpesvirus 1 and caprine herpesvirus 1: viral polypeptides exhibiting shared and unique antigenic determinantsIt is established that strains of bovine herpesvirus 1 (BHV-1) are immunologically closely related, whereas strains of BHV-1 and CapHV-1 (caprine herpesvirus I) display a non-reciprocal immunological relationship. In order to identify viral polypeptides exhibiting shared or unique antigenic determinants a panel of 11 monoclonal antibodies (McAb's) was used to immune precipitate infected cell proteins of three strains of BHV-1 (representing the three established virus types) and one strain of CapHV-1. The McAb's had been generated with a BHV-1/type 1 virus. With the homologous virus the following 13 proteins (p) and glycoproteins (gp) could be identified (mol. wt x 10 7 in parentheses): p3 (153), gp7 (126), gpl0 (102), pll (96), pllA (95), gpl2 (87), p16 (76), gpl7 (73), gpl7A (74), p18 (69), p19 (68), p20 (67), and gp23 (55). The same panel of McAb's precipitated a reduced number of proteins from infected cell extracts obtained with the other ...
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