Lesions of the rat nucleus basalis magnocellularis (nBM) result in a marked decrease in cortical choline acetyltransferase (CAT) and in behavioral deficits. After unilateral ibotenic acid (IBO) lesions of the nBM in rats, there was a significant ipsilateral loss of frontal and parietal CAT, which did not recover for 3 months following surgery and was accompanied by a loss of CAT immunoreactivity in the peripallidal region. Bilateral ibotenate nBM lesions resulted in a marked deficit of one-trial step-through passive avoidance (PA) at 24 hours. Cholinesterase inhibitors including physostigmine, N-ethylaklylphenyl carbamate (RA-6), and N,N-methylethylphenyl carbamate (RA-7) were administered in separate experiments, for 2 days before retrieval testing or for 3 consecutive days during consolidation immediately following training. Nonsignificant improvements in PA latency were produced using 0.32 mg/kg physostigmine and 2.5 mg/kg RA-6 administered before retrieval testing. The results suggest that destruction of cholinergic neurons in the nBM are involved in the PA deficit, but does not exclude the possibility that damage to other neuronal systems may contribute to the observed behavioral deficit.
The effect of bilateral nucleus basalis magnocellularis (nBM) lesions on performance in the Morris water task was examined in the rat, and the ability of anticholinesterase inhibitors to reverse the behavioral deficit was evaluated. Lesions of nBM resulted in a prolongation of escape latency. A spatial probe trial revealed that animals with sham lesions swam a greater percentage of the distance in the platform quadrant; this finding was abolished by nBM lesions. Lesions of nBM produced a nonsignificant increase in both open-field activity and activity-box scores. In Experiment 1, administration of 0.32 mg/kg physostigmine on Day 3 only resulted in a decrease in escape latency. In Experiment 2, in which cholinesterase inhibitors were administered daily for 5 days, 0.32 mg/kg but not low-dose physostigmine or two substituted N,N-alkyl phenyl carbamate cholinesterase inhibitors (RA-6 and RA-7) again improved escape latency on Day 3. Thus it was concluded that nBM lesions impair behavior on the Morris water task and physostigmine shortens escape latency.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.