-the only expedient way to run a biological documentation system is to keep the data recording so simple that no specialists are required for encoding; -a research documentation system will only be attractive to the scientist if it provides both the opportunity to carry out individual searches and also a continuing service in the form of SDI; -a research documentation will only remain up-to-date if the system is controlled by the research department, and if the scientist whose research results are documented continually collaborates in its extension and maintenance. In cooperation with the department

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In the accompanying paper (Bendiak et al., 1989), the separation of a series of oligosaccharides released from asparagine residues of fetuin was described. A series of NMR experiments, which included one- and two-dimensional nuclear Overhauser enhancement, two-dimensional correlation spectroscopy, and two-dimensional relayed-coherence spectroscopy, as well as permethylation analyses, established a Gal beta 1----3(NeuAc alpha 2----6)GlcNAc beta 1----4Man unit common to a series of purified structures. These oligosaccharides contained either three, four, or five glycosidically linked sialic acid residues. The NeuAc residue in alpha 2----6 linkage to GlcNAc gives rise to diagnostic chemical shift perturbations of particular proton signals in the oligosaccharides.

ower (amplitude) CDS has significant potential to contribute to improved understanding of tumor vascularity and blood flow in vivo. 1 However, its inability to quantitate blood flow is a major current limitation. It was the purpose of this study to devise and test a 2D and 3D a-CDS system designed to quantitate tumor vascularity and flow as they correlate with histologic determinations of vascularity. In addition, changes in flow after intravenous infusion of CM-101 were studied as a means to evaluate possible effects (within 1 h) in tumor blood flow. This exotoxin has been reported to affect tumor vascularity in our previously reported study and was used as a means to produce changes in vascularity that might be detected by 3D a-CDS. This study was designed to evaluate the accuracy of a system to quantitate tumor vascularity with amplitude (power) color Doppler sonography twoand three-dimensionally. The vascularity of 20 transplanted murine tumors was determined with quantitated amplitude color Doppler sonography both two-and three-dimensionally and compared to tumor vascularity estimated by histologic examination. Serial examinations were performed 15, 30, 45, and 60 min after the injection of the exotoxin CM-101 and saline solution to assess changes in tumor vascularity. Three-dimensional amplitude color Doppler sonography best depicted the overall vascularity of tumor when compared to histologic estimation of vessel density. However, neither twonor three-dimensional amplitude color power angiography correlated well to the microvessel count, probably a reflection of the difference in the method for vessel quantification using sonographic versus histologic techniques. Three-dimensional amplitude Doppler sonography correlated better with counts of large vessels (> 100 µm) as opposed to small vessels (> 15 µm). Time-activity curves showed no difference in tumor flow at the times measured in the experimental group injected with CM-101 or when compared to saline solutions in either the peripheral or central portions of the tumor. This three-dimensional amplitude color Doppler sonographic system affords global quantification of tumor vascularity and flow that may, in turn, be useful in determining the probability of malignancy (by determination of branching patterns and vessel regularity) or tumor response or both to treatment. KEY WORDS: Color Doppler sonography; Tumor, vascularity; Vascularity, tumor.

An experimental model of human fallopian tubes in organ culture was used to examine the ability of lipopolysaccharide (LPS) of Neisseria gonorrhoeae to damage the fallopian tube mucosa. Gonococcal LPS was purified by hot phenol-water extraction and sequential ultracentrifugation. This LPS was highly lethal for lead-sensitized mice and at a concentration as low as 6 pg/ml reproducibly gelled limulus amoebocyte lysate. Gonococcal LPS damaged fallopian tube mucosa in concentrations as low as 0.015 microgram/ml, a values less than the LPS concentration in organ culture medium surrounding fallopian tube mucosa that was damaged by gonococcal infection. The toxic effect of LPS was neutralized by polymyxin B. Gonococci were shown to elaborate blebs of outer membrane material that is likely to contain LPS. These studies suggest that gonococci elaborate LPS-containing material into their surrounding medium, that the LPS is capable of mediating damage to human fallopian tube mucosa, and that the production of mucosal damage requires the lipid A portion of the LPS molecule.