This paper introduces the first results of dielec-7 tric spectroscopy characterization of glioblastoma cells, measur-8 ing their crossover frequencies in the ultra-high-frequency range 9 (above 50 MHz) by dielectrophoresis (DEP) techniques. Exper-10 iments were performed on two glioblastoma lines U87-MG and 11 LN18 that were cultured following different conditions, in order 12 to achieve different phenotypic profiles. We demonstrate here that 13 the presented DEP electrokinetic method can be used to discrim-14 inate the undifferentiated from the differentiated cells. In this 15 study, microfluidic lab-on-chip systems implemented on bipolar-16 complementary oxide semiconductor technology are used allowing 17 single cell handling and analysis. Based on the characterizations 18 of their own intracellular features, both the selected glioblastoma 19 (GBM) cell lines cultured in distinct culture conditions have shown 20 clear differences of DEP crossover frequency signatures compared 21 to the differentiated cells cultured in a normal medium. These re-22 sults support the concept and validate the efficiency for cell char-23 acterization in glioblastoma pathology. 24 Index Terms-BiCMOS chip, biological cell manipulation, 25 glioblastoma cells, high frequency dielectrophoresis. 26 I. INTRODUCTION 27 G LIOBLASTOMA (GBM) is one of the most frequent and 28 the most aggressive tumors of the central nervous system.
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