Scanning transmission X-ray microscopy was used to chemically image RuO(2) nano-layer coated multi-walled carbon nanotubes (RuO(2)/MWCNT) with a spatial resolution of 30 nm. X-Ray absorption near-edge structure spectroscopy of individual RuO(2)/MWCNTs has been compared with that of MWCNTs and RuO(2) which confirms that RuO(2) interacts with MWCNTs through charge redistribution.

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Summary Fruit ripening is a critical phase in the production and marketing of fruits. Previous studies have indicated that fruit ripening is a highly coordinated process, mainly regulated at the transcriptional level, in which transcription factors play essential roles. Thus, identifying key transcription factors regulating fruit ripening as well as their associated regulatory networks promises to contribute to a better understanding of fruit ripening. In this study, temporal gene expression analyses were performed to investigate banana fruit ripening with the aim to discern the global architecture of gene regulatory networks underlying fruit ripening. Eight time points were profiled covering dynamic changes of phenotypes, the associated physiology and levels of known ripening marker genes. Combining results from a weighted gene co‐expression network analysis (WGCNA) as well as cis‐motif analysis and supported by EMSA, Y1H, tobacco‐, banana‐transactivation experimental results, the regulatory network of banana fruit ripening was constructed, from which 25 transcription factors were identified as prime candidates to regulate the ripening process by modulating different ripening‐related pathways. Our study presents the first global view of the gene regulatory network involved in banana fruit ripening, which may provide the basis for a targeted manipulation of fruit ripening to attain higher banana and loss‐reduced banana commercialization.

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With the global warming slowdown in the twenty-first century, a huge discrepancy in regional climate warming has been identified over the main region of the Tibetan Plateau (TP). Compared with the +0.04 C/decade warming from 1961 to 1999, the warming greatly accelerated for the period 2000-2015 at a rate of +0.30 C/ decade. During the same period, warming in the cold season (November to March) was more pronounced than in the warm season (May to September) over the TP. The results also indicated that the middle-level cloud (middle cloud) decreased (−0.359%/year), while the high-level cloud (high cloud) increased (+0.241%/year) over almost all the TP during the cold season. Further analysis showed positive net cloud radiative forcing over the western TP from 2000-2015, that is, a heating effect of clouds, especially in the cold season. Combining the trends of the increase in high cloud and the decrease in middle cloud over most parts of the TP, the decreased albedo effect of middle cloud and the increased longwave greenhouse effect of high cloud may have partially contributed to the sustained warming, especially in the cold season from 2000 to 2015. Meanwhile, the results showed that the warming rate and cloud area fraction changes were significantly amplified with elevation. The analysis based on a model of Coupled Model Intercomparison Project Phase 5 shows that the decreased middle cloud plays more important role than the increased high cloud in modulating the enhanced warming over the TP, especially in the cold season.

Banana fruit (Musa acuminate L.) ripening is a complex genetical process affected by multiple phytohormones and expression of various genes. However, whether plant hormone brassinosteroid (BR) is involved in this process remains obscure. In this work, three genes that encode BR core signaling components brassinazole resistant (BZR) proteins, namely MaBZR1 to MaBZR3, were characterized from banana fruit. MaBZR1-MaBZR3 exhibited both nuclear and cytoplasmic localization and behaved as transcription inhibitors. Expression analysis showed that MaBZR1/2/3 were continuously decreased as fruit ripening proceeded, indicating their negative roles in banana ripening. Moreover, gel shift and transient expression assays demonstrated that MaBZR1/2 could suppress the transcription of ethylene biosynthetic genes, including MaACS1, MaACO13 and MaACO14, which increased gradually during the banana ripening, via specifically binding to CGTGT/CG sequence in their promoters. Importantly, exogenous application of BRs promotes banana ripening, which is presumably due to the accelerated expression of MaACS1 and MaACO13/14, and consequently the ethylene production. Our study indicates that MaBZR1/2 act as transcriptional repressors of ethylene biosynthetic genes during banana fruit ripening.

Carotenoids are a class of bioactive compounds that exhibit health-promoting properties for humans, but their regulation in bananas during fruit ripening remains largely unclear. Here, we found that the total carotenoid content continued to be elevated along the course of banana ripening and peaked at the ripening stage followed by a decrease, which is presumably caused by the transcript abundances of carotenoid biosynthetic genes MaLCYB1.1 and MaLCYB1.2. Moreover, a ripening-inducible transcription factor MaSPL16 was characterized, which was a nuclear protein with transactivation activity. Transient transformation of MaSPL16 in banana fruits led to enhanced transcript levels of MaLCYB1.1 and MaLCYB1.2 and hence the total carotenoid accumulation. Importantly, MaSPL16 stimulated the transcription of MaLCYB1.1 and MaLCYB1.2 through directly binding to their promoters. Collectively, our findings indicate that MaSPL16 behaves as an activator to modulate banana carotenoid biosynthesis, which may provide a new target for molecular improvement of the nutritional and bioactive qualities of agricultural crops that accumulate carotenoids.