We demonstrate herein the synthesis and modification of magnetic nanoparticles and its use in the immobilization of the lipase. Magnetic Fe 3 O 4 nanoparticles (MNPs) were prepared by simple co-precipitation method in aqueous medium and then subsequently modified with tetraethyl orthosilicate (TEOS) and 3-aminopropyl triethylenesilane (APTES). Silanization magnetic nanoparticles (SMNP) and amino magnetic nanomicrosphere (AMNP) were synthesized successfully. The morphology, structure, magnetic property and chemical composition of the synthetic MNP and its derivatives were characterized using transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FT-IR) analysis, X-ray diffraction, superconducting quantum interference device (SQUID) and thermogravimetric analyses (TGA). All of these three nanoparticles exhibited good crystallization performance, apparent superparamagnetism, and the saturation magnetization of MNP, SMNP, AMNP were 47.9 emu/g, 33.0 emu/g and 19.5 emu/g, respectively. The amino content was 5.66%. The AMNP was used to immobilize lipase, and the maximum adsorption capacity of the protein was 26.3 mg/g. The maximum maintained activity (88 percent) was achieved while the amount of immobilized lipase was 23.7 mg g −1. Immobilization of enzyme on the magnetic nanoparticles can facilitate the isolation of reaction products from reaction mixture and thus lowers the cost of enzyme application.
Outcome Based Education has become the mainstream of educational reform in the United States, Britain, Canada and other countries, and has been fully adopted by the professional certification of engineering education. It is of practical significance to guide the reform of engineering education in China with the idea of Outcome Based Education. In the course of cultivating students majoring in chemical engineering and technology, the biggest characteristic is that they need to transfer from pure theory to engineering. But theoretical knowledge is very different from practical engineering, as if there exists a huge gap that needs to be crossed by a bridge. Through this bridge, students can skillfully use the theoretical knowledge they have mastered to solve practical engineering problems. Chemical design is one of the core curriculum to cultivate students' engineering ability and innovation ability. Aimed at the problems existing in the teaching of chemical engineering design course, the teaching reform of chemical engineering design was discussed from the theory teaching, practice teaching, the computer application in chemical engineering design et al. Thus driving the local undergraduate colleges and universities to complete the application-oriented transformation.
Abstract.A new type of macroporous weakly basic anion exchange resin (HFO D301R) was used to adsorption of naphthalene sulfonic acid in different environments. The effects of temperature, salt concentration and pH value of the solution on HFO-naphthalene sulfonic acid resin adsorption effect were studied The results show that HFO-D301R maximum adsorption capacity of β-naphthalene sulfonic acid can reach 235 mg/g at 10 °C. The increase of salt solution concentration (sodium chloride or sodium sulfate) restrain adsorption of iron composite resin on β-naphthalene sulfonic acid; The pH value of the solution also affect the adsorption capacity of the iron composite resin, and the best result of adsorption was obtained at pH=4.Introduction β-naphthalene sulfonic acid sodium salt (β-salt) producted as dye intermediates results in a large number of β -salt mother liquor. The color of mother liquor is deep because it contains 3% of the β-naphthalene sulfonic acid , 3% of the sulfuric acid and a small amount of Sulphur trioxide. The pH of mother liquor is lower than 3. Today, the main processing technologies have chemical oxidation, membrane separation and extraction [1].These presence methods have many shortcomings, which include high consumption of oxidizing agent; easily polluting the membrane materials whose life is low; high consumption of extraction agent; difficult reextraction process currently, the harmlessness of the β-naphthalene sulfonic acid wastewater cannot effectively realize. At present, the ion exchange resin method which are separated of organic and inorganic matter in aqueous solution have some rarely successful examples [2,3]. However, there is not research report that β-naphthalene sulfonic acid/sulfuric acid/sulfite mixture is adsorpted by weakly basic anion exchange resin composited on hydrated ferric oxide. In this article, the main object of study is macroporous weakly basic anion exchange resin (domestic, D301) as the carrier to compound a kind of new adsorption material according to the literature [4]. which is evaluated by ferric oxide composite resin exchange absorption of naphthalene sulfonic acid in different surroundings : the temperature, pH, salt, etc.
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