A survey for natural entomopathogenic fungi of two spotted spider mite (Tetranychus urticae) adults was made in Erzurum, Turkey, during the period 2006. Tetranychus urticae (65.8%) infected with a strain of the fungus Cladosporium cladosporioides were found. Thirteen isolates of C. cladosporioides were assessed against T. urticae, in a single dose (8 9 10 6 conidia ml -1 ), laboratory bioassay on bean leaflets. The total mortality percentage caused by C. cladosporioides isolates varied from 50.95 to 74.76% and LT 50 values ranged from 2.34 to 3.90 days. The results revealed that isolates of C. cladosporioides were effective against two spotted spider mite. This is the first record of natural infection of T. urticae by C. cladosporioides.
Tuncer S., Eken C. (2013) [2007][2008]. The most prevalent multinucleate anastomosis groups (AG) were AG-4 (85.2%), followed by AG-2 type 1 (7.4%), AG-6 (5.0%), and AG-3 (2.5%). The population of binucleate Rhizoctonia spp. comprised AG-A (82.4%), AG-K (11.8%), and AG-G (5.9%). Rhizoctonia solani AG-3 and AG-6, as well as binucleate Rhizoctonia spp. AG-G and AG-K on pepper (c. annuum) were firstly determined in this study. During both in vitro and in vivo pathogenicity experiments differences in virulence level between R. solani and binucleate Rhizoctonia spp. isolates were observed. Isolates of R. solani AG-2 type 1 and AG-4 were the most virulent, binucleate Rhizoctonia spp. isolates of AG-A were less virulent, whereas binucleate Rhizoctonia spp. isolates of AG-G and AG-K were non-pathogenic.
The small poplar longhorn beetle, Saperda populnea is an important pest of Lombardy poplars (Populus nigra L.) in Turkey. A survey for natural entomopathogenic fungi of S. populnea larvae was made in Erzurum, Turkey, during the period 2004-2005. Larvae (13.5%) infected with a strain of the fungus Beauveria bassiana were found. The pathogenicity of B. bassiana strain 46 was conducted with different concentrations of conidia (10(6), 10(7) and 10(8) conidia/ml) of this isolate on S. populnea larvae. The lowest concentration (10(6) conidia/ml) caused about 56% mortality within 6 days. One hundred percent mortality was achieved after median lethal time (LT(50)) of 4.6 and 4.4 days for 10(7) and 10(8) conidia/ml, respectively. There were no significant differences between median lethal times. This is the first record of natural infection of S. populnea larvae by B. bassiana.
This study was carried out to determine distribution, host plant and biology of Ricania simulans (Walker) (Hemiptera: Ricaniidae) in Eastern Black Sea Region of Turkey between 2009 and 2011. There is no important plant pest in the Eastern Black Sea Region of Turkey except this species. However, population of R. simulans being harmful in many wild and cultivated plant species in both nymphal and adult stages has been increased since 2009. As a result of this study, it was determined that R. simulans has been widespread on coastal areas of Eastern Black Sea Region of Turkey with an extensive host range including elderberry, bean, kiwifruit, wild blackberries, hydrangea, fig tree, alder, cherry laurel, tea tree and grapevine. Nymphs of the pest emerge in the middle of May and it has 5 nymphal stages, the adults emerge early July and started to lay their eggs in early August, overwinter as eggs and gives one generation in a year.
Smooth bromegrass (Bromus inermis Leyss.) is the most common perennial grass species cultivated for forage in North America. During late fall of 2004, smooth bromegrass plants in Lincoln, NE were observed to have brown lesions on leaf midveins that were several centimeters long. Symptomatic leaves were surface disinfested for 1 min in 2% NaOCl and incubated at 25°C on potato dextrose agar (PDA) and water agar. The fungus, Pithomyces chartarum (Berk. & Curt) Ellis, was isolated consistently and identified on the basis of morphological characteristics (1). Colonies were effused and black on PDA. Conidiophores measured 3.5 to 8 × 1.9 to 3.9 μm and were smooth and single. Conidia (7 to 25 × 9.5 to 14 μm) were broadly ellipsoidal, pale brown to dark brown, verrucose with mainly three transverse septa and one to two longitudinal septa. Pathogenicity tests were conducted on 50-day-old plants by spraying with a conidial suspension (2.5 × 105 spores per ml). Control plants were sprayed with sterile water. All plants were kept in a moist chamber (100% relative humidity) for 3 days and then transferred to a greenhouse (25°C, >70% relative humidity, and a 12-h photoperiod). One week after spraying, elongated lesions developed on leaf midveins of inoculated plants from which P. chartarum was consistently reisolated. No symptoms were observed on control plants. While P. chartarum has been described as a saprotroph or a parasite on a wide range of plants primarily in the tropics and subtropics, including the southern United States (2), it was reported previously on B. inermis only in Canada (3). This report expands the distribution and host range of P. chartarum as a pathogen in the United States. References: (1) M. B. Ellis. Dematiaceous Hyphomycetes. Commonwealth Mycological Institute, Kew, Surrey, England, 1971. (2) D. F. Farr et al. Fungal Databases, Systematic Botany and Mycology Laboratory, On-line publication. ARS, USDA, 2005. (3) J. H. Ginns. Compendium of Plant Disease and Decay Fungi in Canada 1960-1980. Res. Br. Can. Agric. Publ. 1813, 1986.
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