SUMMARY A subunit antigenoid vaccine, Ac NFU, (S-) MRC, was used to prevent primary herpes genitalis in 60 subjects considered to be at risk of this infection. There was no evidence of serious local or general side effects.Neutralising antibody responses were detected in 59% and 90% of subjects receiving the low and high doses of vaccine respectively; immunoprecipitating antibody was detected at a lower frequency, namely in 23/o and 43% of subjects receiving the low and high doses respectively. After a mean follow-up period of 18 months none of the vaccinated subjects contracted herpes genitalis after completing the vaccination course.
A mouse monoclonal antibody with complement-independent neutralising activity against cytomegalovirus (CMV) and reactive with the 86 kilodalton (kDa) viral glycoprotein H is described. Neutralisation tests against a range of different strains of CMV showed significant crossreactivity, but clear differences were evident between the two prototype viruses AD169 and Davis, and particularly between AD169 and several low-passage recent clinical isolates; CMV present in urine was neutralised weakly if at all.
Rapp et al. (1964) did not find an enhanced tumorigenicity in BHK 21 cells treated with a multiplicity of 3 PFU SV40 virus per cell. They also showed that SV40-immunized adult hamsters were not more resistant to the transplantability of the virus-exposed cells. Black and Rowe (1965) found a growth stimulatory effect on BHK 21/13 cells after exposure to hypertonic saline and SV40 DNA, but a more intensive clonal study would have been desirable in this case to ensure that the differences found between test and control cultures were not due to heterogeneity and selection from the parental stock of cells.Here we describe the successful transformation of BHK 21/13 cells following their cocultivation with SV40-infected monkey cells.
MATERIAL AND METHODS
Cells(a) BHK 21/13. The origins of the diploid fibroblastic cell line BHK 21, and its derivative clone 13 (C13) have been described (Macpherson and Stoker, 1962). (b) BS-C-I cells, a line derived from African Green Monkey Kidney cells (Hopps et al., 1963), were obtained from Flow
Conjugation of carboxypeptidase G and arginase, two enzymes of therapeutic interest, to a soluble dextran significantly enhanced plasma persistence in normal and tumour-bearing mice. A prolonged decrease in arginine concentrations in plasma of tumour-bearing mice was demonstrated by using the dextran-linked arginase. Gel filtration of dextran-enzyme conjugate showed that enzyme activity co-chromatographed as a single peak with carbohydrate, and enzyme was shown to be covalently linked to the dextran.
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