We conducted a micro-CT analysis of subchondral bone of the vertebral end-plates after application of compressive stress. Thoracic and lumbar vertebral units were instrumented by carrying out left asymmetric tether in eleven 4-week-old pigs. After 3 months of growth, instrumented units and control units were harvested. Micro-CT study of subchondral bone was performed on one central and two lateral specimens (fixated side and non-fixated side). In control units, bone volume fraction (BV/TV), number of trabeculae (Tb.N), trabecular thickness (Tb.Th), and degree of anisotropy (DA) were significantly higher, whereas intertrabecular space (Tb.Sp) was significantly lower in center than in periphery. No significant difference between the fixated and non-fixated sides was found. In instrumented units, BV/TV, Tb.N, Tb.Th, and DA were significantly higher in center than in periphery. BV/TV, Tb.N, and Conn.D were significantly higher in fixated than in non-fixated side, while Tb.Sp was significantly lower. We noted BV/TV, Tb.N, and Tb.Th significantly lower, and Tb.Sp significantly higher, in the instrumented levels. This study showed, in instrumented units, two opposing processes generating a reorganization of the trabecular network. First, an osteolytic process (decrease in BV/TV, Tb.N, Tb.Th) by stress-shielding, greater in center and on non-fixated side. Second, an osteogenic process (higher BV/TV, Tb.N, Conn.D, and lower Tb.Sp) due to the compressive loading induced by growth on the fixated side. This study demonstrates the densification of the trabecular bone tissue of the vertebral end-plates after compressive loading, and illustrates the potential risks of excessively rigid spinal instrumentation which may induce premature osteopenia. Keywords: asymmetric tether; trabecular bone; compression; micro-CT; spineThe pathophysiology of intervertebral disc degeneration has been widely studied.1-3 It has been demonstrated that chronic compression can modify mechanical, histologic, biochemical, and cellular properties of the intervertebral disc.4-7 Moreover, relationships have been established on one hand between disc degeneration and the architecture of trabecular vertebral bone, 8,9 and on the other hand between cell death in annulus and bone mineral density of the vertebral end-plate.10 Moore et al. 9 showed, in an adult ovine model, that an experimental outer annular tear was associated with an increase in the number of trabeculae, while their thickness and the intertrabecular space decreased. The authors linked these changes to alterations in the biomechanical behavior of the local structures. Simpson et al. 8 showed a close relationship between disc degeneration and trabecular architecture of the adjacent vertebral bodies, where bone volume fraction increased with the degree of degeneration. Gruber et al. 10 found a significant correlation between the percentage of cell death in the annulus and the bone mineral density of the vertebral end-plate as estimated by using a densitometer in an aging sand rat model...