The study aimed to develop a non-dairy-based probiotic-supplemented product using an underutilized crop in the Philippines such as the yellow passion fruit (Passiflora edulis f. flavicarpa Deg.). The physico-chemical properties (moisture content, water activity, pH, and total soluble solids), probiotics stability at different storage temperatures (4°C, 25°C, and 37°C), and the sensory characteristics of Lactobacillus plantarum S20-supplemented passion fruit juice powder was evaluated. Passion fruit juice powder and L. plantarum S20 were first prepared using low-temperature spray drying utilizing maltodextrin as a carrier, with yield as 42.97% and 21.17%, respectively. Spray drying of probiotics culture also resulted in 42.68% log survivability. The formulated juice powder had a final moisture content of 1.729±0.38% and water activity of 0.398±0.0051, and with recommended dilution with water, had a final pH and total soluble solids of 3.40±0.10 and 12.00±0.00° Brix, respectively. Results also showed that storage of the formulated juice powder at 4°C yielded the highest probiotic stability, maintaining a viable log count of 4.27 per g, while storage at 37°C showed no microbial growth. Sensory evaluation of probioticsupplemented passion fruit juice against a non-probiotic-supplemented one revealed significant difference in terms of color, sweetness, and sourness, while no significant difference was observed in terms of aroma, mouthfeel, and general acceptability.
The effects of extraction parameters, including temperature (25 – 80 °C), time (30 – 90 min), solvent to sample (S/S) ratio (10 – 50 mL g-1), initial pH (3 - 8) and ethanol concentration (20 – 100%), on the % 2,2-Diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity of kalumpit were screened and optimized using 2-level factorial design and Box-Behnken design (BBD) of experiments. Temperature, S/S ratio, and ethanol concentration exhibited significant effects on the % DPPH radical scavenging activity of kalumpit extract. Response surface models developed for % DPPH and 2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical scavenging activities of kalumpit fruit extract adequately fit and were used to determine the optimum extraction conditions. A desirability function approach determined the optimum conditions for solvent extraction of antioxidants at 80.0 °C, 10 mL g-1 S/S, and 51.66% ethanol concentration. This resulted in a maximum desirability value of 0.977 and predicted % DPPH and ABTS radical scavenging activities of 66.63 and 82.14, respectively. Validation of the adequacy of the predictive models showed no significant difference between experimental data and predicted values (p > 0.05), indicating that the models developed were adequate in describing the relationship between factors and responses.
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