Prostate cancer is ranked second leading cause of cancer death, globally. Conventional treatment strategies hold very limited scope as cancer advances in grade. Treatments such as surgery, radiation therapy and chemotherapy possess adverse side effects which lead us to explore the natural products as anticancer agents. The main aim of present work is to bring together the advantages of ayurvedic formulations with that of modern nanotechnology. Phyllanthus niruri is an ayurvedic plant that has shown its potential as an effective anticancer agent in prostate cancer and its ability as an effective apoptosis inducer as well as an antimetastatic agent in cancer cells. In order to increase the bioavailability of P. niruri extract, stable nanoparticles were prepared and its effect was studied. Size analysis by DLS and SEM revealed the average size of nanoparticles prepared was 150 ± 50 nm. In vitro haemolysis and coagulation assay confirmed the blood biocompatibility of the nanoparticles. In vitro cytotoxicity showed concentration and time dependent toxicity on prostate cancer cells with cell viability of 35% with maximum concentration in 48 hrs.The prepared nanoparticles showed a significant decrease in clonogenity and wound healing capacity. On the whole the above characteristics of P. niruri nanoparticles make it a valuable candidate for prostate cancer therapy.
Introduction: Failure of the timely and orderly reparative process to establish the anatomic and functional integrity results in chronic wound. Inflammation is essential for normal wound healing, but an unchecked inflammation hampers the healing process. Any agent that effectively reduce excess inflammation can be useful in wound healing. The objectives of this study were to evaluate the efficacy of topical curcumin in healing of the chronic wounds and also to study its effect on NF-kB and COX-II expression levels in chronic wound tissues compared to controls receiving standard treatment.
Material and Methods:This is a pilot study conducted in 20 patients with chronic ulcer, divided into two equal groups. A comparision was done between two groups who were treated with topically applied curcumin ointment versus our standard treatment protocol for wound management. All these patients were evaluated clinically, qunatitative bacterial culture and expression of molecular markers in the wound.
Results:The efficacy of curcumin on wound contraction rate, accrural of granulation tissue, epithelizartion and reduction in bacterial colony count was not statistically significant compared to standard treatment protocol. Expression levels of proinflammatory genes mainly COX-2, TNF-α, IL-6, IL-1b was reduced and NF-κB translocation into the nucleus was also inhibited in curcumin treated group compared to standard treatment protocol.
Conclusion:Although there was no difference in both the groups in its clinical parameters, curcumin treated group showed underexpression of pro-inflammatory genes and inhibition of translocation of NF-κB which have a barrier role on the excess inflammation. This favours the positive role of curcumin on treating the chronic wounds.
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