Effect of Baliospermum montanum nanomedicine apoptosis induction and anti-migration of prostate cancer cells

Cherian, Aleena Mary; Snima, K. S.; Kamath, C. Ravindranath; Nair, Shantikumar V.

doi:10.1016/j.biopha.2015.02.016

Cited by 19 publications

(13 citation statements)

References 19 publications

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“…The nano-sized benzofuran–pyrazole BZP-NPs of different sizes (3.8–5.7 nm) were synthesized using the nanoprecipitation method [18]. The sizes and morphology of the nanobenzofuran–pyrazole hybrid BZP-NPs were examined by dynamic light scattering (DLS) and transmission electron microscopy (TEM).…”

Section: Resultsmentioning

confidence: 99%

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A Comparative Study of the Anticancer Activity and PARP-1 Inhibiting Effect of Benzofuran–Pyrazole Scaffold and Its Nano-Sized Particles in Human Breast Cancer Cells

et al. 2019

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Breast cancer is considered the most common and deadly cancer among women worldwide. Nanomedicine has become extremely attractive in the field of cancer treatment. Due to the high surface to volume ratio and other unique properties, nanomaterials can be specifically targeted to certain cells and tissues to interact with the living systems. The strategic planning of this study is based on using the nanoprecipitation method to prepare nanoparticles BZP-NPs (3.8–5.7 nm) of the previously prepared benzofuran–pyrazole compound (IV) BZP which showed promising cytotoxic activity. The capacity of BZP and BZP-NPs to suppress the growth of human breast tumor MCF-7 and MDA-MB-231 cells was evaluated using MTT assay. The IC50 doses of BZP and BZP-NPs targeting normal breast cells MCF-12A exceeded those targeting the cancer cells by >1000-fold, demonstrating their reasonable safety profiles in normal cells. Furthermore, cell cycle analysis, apoptosis induction detection, assessment of p53, Bcl-2, caspase-3, and PARP-1 levels of BZP and its nano-sized-BZP-NPs particles were also evaluated. Although the obtained results were in the favor of compound IV in its normal-sized particles, BZP-NPs appeared as a hit compound which showed improved cytotoxicity against the tested human breast cancer cells associated with the induction of pre-G1 apoptosis as well as cell cycle arrest at G2/M phase. The increase in caspase-3 level, upregulation of p53, and downregulation of Bcl-2 protein expression levels confirmed apoptosis. Furthermore, ELISA results exhibited that BZP-NPs produced a more favorable impact as a PARP-1 enzyme inhibitor than the parent BZP.

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Section: Resultsmentioning

confidence: 99%

“…The nanoparticles were prepared by the nanoprecipitation method [18]. The sizes and morphology of the nanoparticles BZP-NPs were examined by transmission electron microscopy (TEM) (H-7600; Hitachi Ltd., Tokyo, Japan).…”

Section: Methodsmentioning

confidence: 99%

A Comparative Study of the Anticancer Activity and PARP-1 Inhibiting Effect of Benzofuran–Pyrazole Scaffold and Its Nano-Sized Particles in Human Breast Cancer Cells

et al. 2019

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“…Baliospermum montanum is an anticancer plant used in Ayurvedic medicine [167]. Nanoparticles prepared with both aqueous and ethanolic extracts of B. montanum presented a dose and time-dependent toxicity on prostate cancer cells with cell viability of 22% and 6% with a maximum concentration of aqueous and ethanolic nanoparticles (2 mg/mL), respectively, in 48 h. Also, no in vitro hemolysis and significant reduction of the wound healing capacity and colony forming ability of the prostate cancer cells was demonstrated [121]. Cnidoscolus chayamansa plants showed cytotoxic activity on LNCaP with a percent inhibition greater than 50% [123].…”

Section: Plant Extracts and Plant-derived Bioactives In Prostate Cmentioning

confidence: 99%

Phytochemicals in Prostate Cancer: From Bioactive Molecules to Upcoming Therapeutic Agents

et al. 2019

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Prostate cancer is a heterogeneous disease, the second deadliest malignancy in men and the most commonly diagnosed cancer among men. Traditional plants have been applied to handle various diseases and to develop new drugs. Medicinal plants are potential sources of natural bioactive compounds that include alkaloids, phenolic compounds, terpenes, and steroids. Many of these naturally-occurring bioactive constituents possess promising chemopreventive properties. In this sense, the aim of the present review is to provide a detailed overview of the role of plant-derived phytochemicals in prostate cancers, including the contribution of plant extracts and its corresponding isolated compounds.

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“…Collectively, recent updates on green nanomedicine have been documented in an area of prostate cancer specific theragnosis [32]. In contrast, our group developed a simple, non-toxic, and efficient phytoactive nanomedicines such as boswellic acid in vitro [33], in vivo [34], Phyllanthus niruri [35], Tinospora cardifolia [36], silymarin [37], Baliospermum montanum [38], Plumbago zeylanica [39], and Leucas aspera [40] have been demonstrated as a therapeutic potential of those nanomedicines agent for prostate cancer. In the search of extended novel natural products for their efficacy against prostate cancer, we investigated Biophytum sensitivum (BS) is a medicinal plant traditionally used to cure various diseases.…”

Section: Introductionmentioning

confidence: 99%

Biophytum sensitivum nanomedicine reduces cell viability and nitrite production in prostate cancer cells

Raju

Nair

2017

IET nanobiotechnol.

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Phytomedicine research received tremendous attention for novel therapeutic agent due to their safety and low cost. We assessed a novel nanoformulation of Biophytum sensitivum (BS), natural flavonoids for their improved efficacy and superior bioavailability against crude extract for prostate cancer cells (PC3). We prepared a nanomedicine of BS by nanoprecipitation method and size analysis via DLS and SEM revealed a range of 100-118 nm and surface zeta potential as −9.77 mV. FTIR was performed to evaluate functional for presence of carbonyl and aromatic rings, respectively. Human PC3 cells showed concentration at 0.5, 0.8, and 1 mg/ml dependent cytotoxicity 22, 39, and 56% for 24 h, whereas 43, 41, and 67% for 48 h of BS nanomedicine compared with crude 11, 22, and 53% for 24 h and 38, 31, and 60% for 48 h, respectively. Haemocompatibility of BS nanomedicine at the concentration of 0.5, 0.8, and 1 mg/ml did not show blood aggregation. Cellular uptake was confirmed using rhodamine-conjugated BS nanomedicine for 48 h. Interestingly, BS nanomedicine 1 mg/ml decreases the nitrite productions in PC3 cells. Collectively, BS nanomedicine has the potential anti-cancer agents with biocompatible and enhanced efficacy can be beneficial for the treatment of prostate cancer

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Effect of Baliospermum montanum nanomedicine apoptosis induction and anti-migration of prostate cancer cells

Cited by 19 publications

References 19 publications

A Comparative Study of the Anticancer Activity and PARP-1 Inhibiting Effect of Benzofuran–Pyrazole Scaffold and Its Nano-Sized Particles in Human Breast Cancer Cells

A Comparative Study of the Anticancer Activity and PARP-1 Inhibiting Effect of Benzofuran–Pyrazole Scaffold and Its Nano-Sized Particles in Human Breast Cancer Cells

Phytochemicals in Prostate Cancer: From Bioactive Molecules to Upcoming Therapeutic Agents

Biophytum sensitivum nanomedicine reduces cell viability and nitrite production in prostate cancer cells

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