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In order to evaluate the clinical-laboratorial alterations, six Nellore calves were inoculated with 10 7 Trypanosoma vivax isolated from Poconé region, Mato Grosso, Brazil. The animals were evaluated daily for rectal temperature, packed cell volume (PCV), parasitemia, antibody production, color of mucous membranes, behavior and appetite. Blood and serum samples for biochemical evaluation for aspartate aminotransferase (AST), alkaline phosphatase (AF), gamma glutamyltransferase (GGT), cholesterol, urea, creatinine, creatine kinase (CK), calcium, phosphorus and proteinogram were collected on days 4, 8, 12, 16, 23 and 30 post inoculation (DPI). During the following 6 months rectal temperature, PCV and parasitemia were evaluated weekly. T. vivax was evidenced from 1 DPI in all calves and persisted until day 30 in five of six animals. A remarkable decrease (p<0.05) of PCV mean value (25%) was observed on 10 DPI. The animals presented no alterations in their clinical or serum biochemical state during the trial. Seroconversion took place 6 and 8 DPI, and all the animals remained seropositive during the 30 days of experiment. In all the experimental animals the occurrence of T. vivax infection was verified, characterized by the increase of corporal temperature, presence of the blood protozoa and reduction of the globular volume, without alterations in the other variables analyzed. Nellore calves, when experimentally inoculated with T. vivax, are able to establish a balance between host-parasite relationship.

A rapid indirect enzyme-linked immunosorbent assay (ELISA) was developed for measuring antibodies against Anaplasma marginale using a partially soluble antigen prepared from semi-purified initial bodies from erythrocytes with 80.0% of rickettsiaemia. This technique utilized alkaline phosphatase and p-nitrophenyl phosphate as reaction indicators. The high sensitivity (100.0%) was confirmed with sera from 100 calves experimentally-infected with A. marginale. All of these animals showed seroconversion before or at the same time of the first rickettsiaemia or even when it was not detected. Also the elevated specificity (94.0%) was confirmed by the low percentage of cross-reactions with sera from animals experimentally-infected with Babesia bigemina and Babesia bovis (1.4 and 6.6%, respectively). Performances of ELISA and indirect fluorescent antibody test (IFAT) with 324 sera from enzootically stable area did not show statistical difference (P>0.05), since the former showed 96.9% and the latter 97.2% of positive reactions. The advantage of this ELISA is a shorter execution time than others developed until now, allowing more samples to be analyzed.

The Ripollesa sheep breed is an important Spanish local breed mainly located in the Mediterranean mountains and cultivated plains of Catalonia, exploited under semi-extensive production systems and currently intended for meat production. The morphological characterization of the Ripollesa breed was carried out from the study of eight flocks representative of different subpopulations. A total of 224 Ripollesa ewes and 17 rams were recorded for their live weight and 12 morphological measures, which were used for calculating 12 zootechnical indexes. Differences (P < 0.05) between flocks and sexes were revealed for several measures. A principal component analysis was performed on the morphological measures and revealed two main principal components accounting for 47.6 and 12.3 percent of the inertia, being related to the frame (body size and live weight) and to withers height and ear length, respectively. The cluster analysis allowed differentiating among four subpopulations with relevant implications to be taken into account for the breed conservation programme. The observed differences could be attributable to geographic location, selective history, flock management and genetics. The estimated zootechnical indexes classified the Ripollesa sheep breed as a medium-sized and long-shaped body frame, with a marked orientation to meat production and with signs of adaptation to the environment.