No abstract

Antioxidant and cytoprotective activities of boiled, cold, and methanolic extracts of nine edible vegetables in Southwest Nigeria were evaluated in the 1,1-diphenyl-2-picrylhydrazyl free radical assay and hemagglutination assay in bovine erythrocytes, respectively. Crassocephalum rubens showed the highest antioxidant activity (56.5%), Solanum americanum and Vernonia amygdalina exhibited moderate antioxidant activity (26.0-37.5% and 14.8-36.2%, respectively), Solanum macrocarpon, Telfaria occidentalis, Amaranthus hybridus, and Jatropha tanjorensis produced weak activity (1.6-15.8%, 1.6-7.7%, 2.8-6.62%, and 10.7-12.1%, respectively), while Celosia argentea and Talinum triangulare were pro-oxidants. It was also shown that extracts from all the vegetables are pro-oxidants at high concentrations of either 1 or 5 mg/mL or both. On the other hand, the studies on the cytoprotective effect showed that all the plant extracts demonstrated a very low hemagglutination titer value between 0.32 and 5.56 except S. americanum methanolic extract, which had a titer of 50.0. These results indicated correlation between the antioxidant properties and the hemagglutination values of these plant extracts; however, the membrane stabilizing capacity of the extracts supports the plants' antioxidant activity.

Dorstenia barteri and D. convexa extracts and some isolated components of the former were investigated for effectiveness against Trichomonas gallinarum and compared with quercetin and quercitrin. The antioxidant activity of the extracts/compounds was also determined. The minimum lethal concentrations (MLCs) for the extract of D. barteri leaves and twigs at 24 h were found to be 15.625 and 15.625 µg/ml, respectively. However, the MLCs of the leaf and twig extract of D. convexa were 125 and 437.5 µg/ml, respectively. The prenylated and geranylated chalcones were as active as the prenylated flavones, 6-prenylapigenin and the diprenylated derivative 6,8-diprenyleridictyol. The order of the antitrichomonal activity of the compounds at 24 h was: quercetin (0.121 µg/ml) > quercitrin (0.244 µg/ml) ≥ bartericin B (0.244 µg/ml) > bartericin A (0.73 µg/ml) > stigmasterol (0.98 µg/ml) > 6,8-diprenyleridictyol = isobavachalcone = dorsmanin F (31.25 µg/ ml). D. barteri extracts, quercitrin, and bartericin A, and the prenylated flavonoids had potent antioxidant properties. The twig extract of D. barteri was more potent than the leaf extract. Moderate (EC 50 >50 µg/ ml) and high (EC 50 <50 µg/ml) antioxidant activities were detected in the leaf and twig extracts of D. barteri and the prenylated flavonoids. Prenylated flavonoids and the isolated compounds with antioxidant properties described here may account for the anti-inflammatory action of these extracts. The antitrichomonal and antioxidant activities shown by the extracts and compounds in this study are consistent with the ethnomedicinal and local use of the Dorstenia species studied.