Using gradient polyacrylamide gel electrophoresis (gradient between 10-20%) we were able to show up to six clearly distinguishable ADH-bands in Hansenulu polymorphu CBS 4732. In contrast t o commercial ADH from Saccharomyces cerevisiae the ADH-forms of Hamenula polymorpha CBS 4732 have a much broader substrate specificity. Furthermore, the occurence of secondary alcohol dehydrogenases and of an aromatic alcohol dehydrogenase could be demonstrated.
The occurence of multiple forms of alcohol dehydrogenase (ADH) in methylotrophic yeasts is known from the literature. But no systematic work has been done to characterize these enzymes in detail. The aim of this work was to establish a suitable electrophoretic method for visualization of the ADH-patterns of these organisms. It was found that the recovery of the enzyme activity during polyacrylamide gel electrophoresis (PAGE) depends on the composition of the gel buffer.The ADH of five different methylotrophic yeasts from the genera Candida, Pichkz and Hansenula were analyzed. Every investigated organism shows rather complex ADH-patterns.
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