Sucrose density-gradient analysis is one method of identifying specific estrogen and progesterone receptors in mammary tumor tissue. Use of the vertical rotor makes this practical for routine applications in the clinical laboratory by increasing the number of samples that can be run at one time and shortening the centrifuge time from 18 to less than 2 h. The separations and reproducibility compare favorably with those obtained with a swinging-bucket rotor.
A simple automated procedure for total serum cholesterol, employing a stable Liebermann-Burchard reagent, was tested. The method has good precision, and results agree well with extraction methods for total cholesterol on normal serums. Serum samples with elevated bilirubin levels gave cholesterol values which were too high and which could not always be corrected for. Lipemic serums in some instances gave results which were too low when compared to other methods.
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