Effect of Bilirubin and Lipemia on an Automated Method for Serum Cholesterol

Abstract

A simple automated procedure for total serum cholesterol, employing a stable Liebermann-Burchard reagent, was tested. The method has good precision, and results agree well with extraction methods for total cholesterol on normal serums. Serum samples with elevated bilirubin levels gave cholesterol values which were too high and which could not always be corrected for. Lipemic serums in some instances gave results which were too low when compared to other methods.