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In welcoming participants to Giant Prawn 2011, the authors briefly review the current scale of this component of global aquaculture. The expansion of freshwater prawn farming over the 30 years since the first global conference on this topic [Giant Prawn 1980] is examined. At the time of GP1980, the output of farmed giant river prawns (Macrobrachium rosenbergii) was <3000 t. Almost three decades later (2009) the total annual aquaculture production of all species of freshwater prawns had risen to almost 444 000 t, with a value of US$2.2 billion. The farmed production of M. rosenbergii constituted 51.7% of the global total, while the oriental river prawn M. nipponense (reared totally in China) contributed 47.2%. The contribution of the monsoon river prawn M. malcolmsonii remains quite small so far, and does not show in the above percentages because no recent FAO data are available for this species. In 2007, however, the latter species contributed 1.0% of the total global production of freshwater prawns. The major freshwater prawn producing countries are in Asia (e.g. Bangladesh, China, India, Myanmar, Taiwan, Thailand and Vietnam) but Macrobrachium spp. are also farmed in other continents.

Seahorses (Hippocampus spp.) are a major commodity fished from the shallow coastal seas of the south coast of India where there is an abundance of sea grasses, sponges and corals. They are in great demand for export as traditional medicines, curios and aquarium fish. Organised fishing and trade of seahorses exists in India along the Palk Bay and Gulf of Mannar coasts. At the Palk Bay coast, seahorses are targeted by divers along with sea cucumbers (Holothuria spp.) and gastropods (e.g. Murex spp., Xancus pyrum Hornell). In the Gulf of Mannar, most of the seahorses are landed as bycatch of shrimp trawling. Seahorses are also fished from Kerala as a bycatch of trawling, although no organised fishery and trade exists. Five species of seahorses were identified from the Palk Bay coast, whereas only two species were obtained from Kerala. Most seahorses from India are exported to Singapore, Hong Kong, Malaysia and the United Arab Emirates. The volume of dried seahorse trade from India was estimated to be 9.75 t as derived from catch data in 2001, which was much higher than official statistics of 4.34 t during 2001-2002, suggesting the major part of the exports might be through non-conventional means and goes undeclared. Some aspects of the impact of large-scale fishing and trade on conservation of these seahorses are discussed.

Freshwater prawn production in India that includes farming and wild capture of the giant freshwater prawn, Macrobrachium rosenbergii and the monsoon river prawn, M. malcolmsonii has increased steadily since 1999 reaching a peak output of 42 780 t in 2005, but then declined to 6568 t in 2009-2010. Stunted growth and diseases in ponds because of poor seed quality and the broodstock which had been inbred over several generations; pond water quality issues; and increased cost of production on account of feed, labour and the mandatory certification requirements are suggested to be some of the factors leading to the production declines. While majority of the output occurs in Andhra Pradesh, single crop paddy-prawn production systems in the low-lying fields of Kerala have helped gradual transformation to a sustainable, organic mode of farming of both rice and prawns, suitable for other states of India. Although the trends by June 2011 indicate that the sector is set to a revival, future prospects of freshwater prawn farming in India will also depend on the expansion of whiteleg shrimp Litopenaeus vannamei that was introduced recently in India and provided a more profitable opportunity for farming.

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The standardisation and optimisation of a one step single tube reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) procedure is described for rapid diagnosis of white tail disease, a viral disease caused by Macrobrachium rosenbergii noda virus (MrNV) and extra small virus (XSV), in giant fresh water prawn, M. rosenbergii. Time, temperature and quantity of each reagent were optimised for the detection of the two viruses. This method was more sensitive than the conventional reverse transcriptase polymerase chain reaction (RT-PCR) for detecting the two viruses. The RT-LAMP reaction is highly suited for disease diagnosis in developing countries. Amplification of DNA can be detected without the use of agarose gel electrophoresis, by the production of a whitish precipitate of magnesium pyrophosphate as a by-product. The cost of RT-LAMP for one reaction is nearly 4 times less than that of RT-PCR.

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