In this paper we describe an initial reproducibility study of 12 proprietary compounds followed by the assessment of 51 marketed pharmaceuticals and, lastly, a summary of the data so far from 2698 proprietary compounds from the Johnson & Johnson (J&J) compound library, in the yeast GreenScreen assay (GSA). In this assay, a reporter system in the yeast cells employs the DNA damage inducible promoter of the RAD54 gene, fused to the extremely stable green fluorescent protein (GFP). The assay proved to be very robust, the Excel templates provided by Gentronix with the assay interfaced well with in-house J&J systems with little adaptation, the assay was very rapid to perform and used very little compound. The results confirm previous work which suggests that the yeast GSA detects different classes of genotoxic compounds to the Ames assay and as a result can help screen out important genotoxic compounds at the pre-regulatory test phase that are missed by Ames-test-based screens alone. A combination of SAR evaluation of genotoxicity plus an Ames-test-based screen and the GSA provides a powerful pre-regulatory test battery to aid in the selection of successful drug candidates.
We report on two sibs with facial anomalies and developmental delay. Partial trisomy 2q was detected only after parental chromosome studies showed the father to carry a balanced interchromosomal insertion of 2 (q24.3-q32.1) into 5q.
We report on two sibs with facial anomalies and developmental delay. Partial trisomy 2q was detected only after parental chromosome studies showed the father to carry a balanced interchromosomal insertion of 2 (q24.3-q32.1) into 5q.
Summary
One set of case notes a week for 12 successive weeks was selected randomly for each of twenty‐eight house officers and scored for use of the POMR format in the data base, initial plans, and progress notes. Analysis was based on 336 sets of records. The purpose of the study was to obtain information which might improve the techniques of teaching POMR to junior hospital staff.
The significant findings were: marked differences in scores among house officers; those who performed well or badly did so in all three components of the system; high scorers in the medical group identified more problems with no evidence that their patients were more ill; scores were better for house officers with favourable attitudes toward POMR and scores were better in the innovative sections if senior staff used the system; house officers with BA or BSc degrees scored better with initial plans and progress notes. No positive effect of feedback was demonstrated.
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