“…The promoter sequences of RAD51, RAD54, HUG1, RNR2, and RNR3 have been used in reporter plasmids to detect agents that damage DNA, and assay strains have been shown to respond to various genotoxic agents such as gamma ray, methyl methanesulfonate, 4-nitroquinoline-1-oxide, methyl-Nnitro-N-nitrosoguanidine, cisplatin, and camptothecin (Walmsley et al, 1997;Billinton et al, 1998;Afanassiev et al, 2000;Boronat and Piña, 2006;Benton et al, 2007;Liu et al, 2008;Wei et al, 2013). The RAD54-GFP reporter assay strain has been validated as a genotoxicity test called the GreenScreen Assay (Cahill et al, 2004;Walsh et al, 2005;Van Gompel et al, 2005;Knight et al, 2007). The promoter sequences of the CUP1, SEO1, and UFO1 genes have been used to detect heavy metals.…”