Microsatellite analysis and genotypingGenomic DNAs were isolated following a conventional method (Sambrook et al., 1989). Sixty five microsatellite markers on chromosomes 1, 2, 11, 13, 17 and 18 were used in the PCR amplification, of which 47 were polymorphic in the founder generation (table 1). These microsatellite markers gave a reasonable coverage of these 6 chromosomes, which belonged to one of three types of swine chromosomes. Chromosomes 1 and 2 were submetacentric, 11 was metacentric, and 13, 17 and 18 were acrocentric.PCR analysis of microsatellites was carried out in an ABI PRISM TM 877 integrated thermal cycler (Perkin-Elmer, USA) using fluorescently labeled PCR primers provided by
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