The marked differences in the morphology of variants of Pseudomonas aerunosa make it abundantly clear that the practice of recording a single morphological description for this species is no longer tenable. It was shown previously, using the smooth state and a flocculent variant of this organism, that recognizable morphological characteristics could be correlated with virulence and with resistance to bactericidal agents (Gainor and Williamson, 1951). It was therefore decided to compare the morphological and physiological characteristics of cells in the mucoid form with those of the smooth state and its filamentous variant. MATERIALS AND METHODS Isolation and characterization of colonial variants. The colonies of the original culture of P.
Certain straimts of Streptococcus pyogenes, Type 12 elaborate a polypeptide which is miephritogemuic imi experimemutal ammimals. It appeared to be a pmmre, umumumigratimig compommemut by paper chronuatography, to be electrophoretically pure whert analyzed by the Spimuco electrophoretic system, amid to be homogemueoums by umltracemmtrifugal amialysis. Quamttitative amino acid ammalyses of acid-hydrolyzed samples revealed 19 amimmo acids amtd trace amoummits of five unidentified compoumemits. Glycimme, proline, alammimie, glutamic acid and aspartic acid accoumited for 70% of the amimino acids foumid. Wilzbach tritiated samples were immjected imttraperitoneally into 20-g C57B1 male mice. Radioautographic evidemmce revealed that a high percemttage of the imtjected material comicemitrated in renal tissue. Certain strains of Streptococcus pyogenes, Type 12, elaborate a mmuixture of polypeptides which is nephritogenic in exl)erimental animals (2, 5). In the)oly)e)tide mixture 14-15 components were found, and one of these appears to be es-)e(ially important to initiation of the neh)hritic syndrome (6). 'l'hese investigations were directed toward evaluating the purity of the isolated)0ly)e)tide, determining its amino acid composition amid finally, estal)hshing whether it localizes in significant concentrations in the kidney. The latter tmssumiies immuh)ortance i)ecause investigations of acute glomerulonephritis, carried on in our lai)oratoly, are i)ased upon the hypothesis that the haptenic Polyl)el)tide (onj uigates to renal protein yielding an antigenic comnplex. MATERIALS ANI) IIuETHOm)s Cultures: A nort-rmephritogemmic Type 6 and a muephuritogenic Type 12 , st raimm II-8 Streptococcus pyogenes were very kindly semmt to us by 1)r. Roger Reed of McGill University (4). The nephritogeuiic qumality of our lyophile stock of the Type 12 culture was comufirmed imu several dozen C57B1 nmice before the orgammisnis were used in more elaborate experinuemits. After rout imme morphological and cultural stuidies amud the comufirmation of teristics of a nephnitogemuic streptococcal polypeptide.
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