Treatment of Experimental Streptococcal Nephritis I. A Preliminary Report

Williamson, C. K.

doi:10.1002/jps.2600500407

Cited by 2 publications

(1 citation statement)

References 9 publications

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“…Matheson and Reed have demonstrated that certain nephritogenic type 12 strains produce nephrotoxic polypeptides which induced acute, subacute, and chronic glomeruloilephritis in rabbits (9) and monkeys (17). Their rabbit experiments (15,16) have been confirmed by Becker and Murphy (I), who used infection, and by Williamson (22), who used type 12 polypeptides.…”

Section: Introductionmentioning

confidence: 61%

Experimental nephritis due to type specific streptococci. V. Attempted use of tissue cultures and mice for the assay of nephrotoxic streptococcal polypeptides

Franklin¹,

Matheson²,

Reed³

1969

Can. J. Microbiol.

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Because the assay of streptococcal polypeptides for nephrotoxic activity has so far depended on extensive and time-consuming rabbit experiments, alternative shorter bioassay methods were sought. Rabbit kidney cell monolayers were exposed to nephrotoxic type 12 and non-nephrotoxic type 6 streptococcal filtrates, dialysates, and polypeptides for varying periods; the latter were in doses of 1–10 mg per tube. In all cases both type 12 and type 6 products proved to be cytotoxic to the monolayers, although, in rabbits, only type 12 products were nephrotoxic. Injection of mice with similar products in varying doses and by varying routes of administration failed to distinguish between nephrotoxic and non-nephrotoxic preparations in terms of renal damage, for all preparations produced similar renal changes. It must be concluded that neither rabbit kidney cell monolayers nor mice can replace rabbits for bioassay of the nephrotoxic potency of streptococcal products.

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Section: Introductionmentioning

confidence: 61%

Experimental nephritis due to type specific streptococci. V. Attempted use of tissue cultures and mice for the assay of nephrotoxic streptococcal polypeptides

Franklin¹,

Matheson²,

Reed³

1969

Can. J. Microbiol.

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Lactic Dehydrogenase Studies in Animals Infected With Type 12 Streptococci or Treated With Streptococcal Nephrotoxin

Elliott¹,

Williamson²

1967

Can. J. Microbiol.

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A histochemical and biochemical study of the reactions of Wistar rats and New Zealand rabbits to Streptococcus pyogenes, type 12, strain H-8, and the nephrotoxin (NT) elaborated by these organisms is presented. A significant lactic dehydrogenase (LDH) increase was found in the sera of all test rats 27 days following treatment with streptococcal NT. However, before this increased activity was detected, a marked decrease in LDH activity was obtained as early as 1 day after the last NT injection. This decrease was modest in rats treated with 1.0 mg NT, but it was great when the dosage of NT was increased to 5.0 mg. Serum LDH values also decreased progressively, up to about 2 weeks, in rabbits infected with nephritogenic streptococci or treated with streptococcal nephrotoxin. Such serum LDH fluctuations did not occur in animals treated with saline or Todd–Hewitt broth. Histochemical studies also suggested an initial inhibition by NT of LDH activity in renal tissue.

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Treatment of Experimental Streptococcal Nephritis I. A Preliminary Report

Cited by 2 publications

References 9 publications

Experimental nephritis due to type specific streptococci. V. Attempted use of tissue cultures and mice for the assay of nephrotoxic streptococcal polypeptides

Experimental nephritis due to type specific streptococci. V. Attempted use of tissue cultures and mice for the assay of nephrotoxic streptococcal polypeptides

Lactic Dehydrogenase Studies in Animals Infected With Type 12 Streptococci or Treated With Streptococcal Nephrotoxin

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