The study investigated the effect of chop length of wheat straw in a high-straw dry cow diet on the feeding behavior, health, and performance of Holstein dairy cows across the transition period. Forty cows were enrolled at dry off, approximately 45 d before expected calving, and assigned to 1 of 2 dietary treatments containing (on a dry matter basis) 36% corn silage, 35% dry cow pellet, and 29% wheat straw. Diets were formulated to target an energy consumption of 19 Mcal/d of net energy for lactation per cow. The diets differed in the chop length of wheat straw: (1) long straw chopped with a 10.16-cm screen (LDD; n = 20), or (2) short straw chopped with a 2.54-cm screen (SDD; n = 20). Dietary treatments were fed throughout the dry period. Feeding behavior, rumination time, and reticulorumen pH were monitored electronically, and feed samples were collected to determine feed sorting. Blood samples were taken weekly, and body condition scores and body weights were taken every 2 wk. Postcalving, all cows were fed the same lactating cow total mixed ration for 28 d; the same measurements were recorded with the addition of daily milk yield and twice weekly milk components. During the dry period, cows fed the SDD treatment consumed more dry matter (15.6 vs. 15.0 kg/d), had more consistent dry matter intake in the week leading up to calving, had a faster feeding rate, consumed 0.4 kg/meal more, and had 0.6 fewer meals per day. No differences were observed in rumination time or mean reticulorumen pH between treatments in both the dry and lactating period. Regardless of treatment during the dry period, cows sorted against the long particles (>19 mm) and in favor of the short particles (<8, >4 mm), but cows fed the LDD treatment sorted to a greater extent than cows fed the SDD treatment. Dietary treatment in the dry period did not influence feed sorting behavior postcalving. Cows fed the LDD treatment had a greater daily change (decline) in rumen pH in the first week postcalving and had a greater blood β-hydroxybutyrate concentration in the third week postcalving. Overall, the results from this study indicate that feeding shorter chopped wheat straw in a high-straw dry cow diet improved intake, reduced sorting during the dry period, and resulted in greater metabolic health and rumen stability in early lactation.

The objective of this study was to determine the effects of adding molasses-based liquid feed to a highstraw dry cow diet on intake, behavior, health, and performance of dairy cows across the transition period. Holstein cows (n = 40) entering their second lactation or greater were enrolled at dry-off (~45 d before expected calving) and assigned to 1 of 2 treatments: a high-straw dry cow total mixed ration (TMR) with either (1) no molasses [CON; n = 20; 10.7% crude protein (CP), 1.45 Mcal/kg of net energy for lactation (NE L )] or (2) supplemented with 1 kg/cow daily [dry matter (DM) basis] of molasses-based liquid feed (LF; n = 20; 11.2% CP, 1.49 Mcal/kg of NE L ). At calving all cows were fed the same lactating TMR (14.9% CP, 1.68 Mcal/kg of NE L ) and followed for 28 d. During the dry period, cows fed the LF diet had greater DM intake (15.3 ± 0.24 vs. 13.4 ± 0.24 kg/d) and tended to have a shorter interval between meals (192.8 ± 8.38 vs. 216.5 ± 7.97 min) and more frequent meals (6.3 ± 0.27 vs. 5.6 ± 0.27 meals/d), while consuming their feed faster (0.09 ± 0.001 vs. 0.08 ± 0.001 kg of DM/ min) compared with cows fed the CON diet. Regardless of treatment, cows sorted [100% × (actual intake/predicted intake)] against the longest ration particles (>19 mm), with cows fed the CON diet sorting more against these (81.1 ± 2.6 vs. 93.9 ± 2.6%). Cows fed the CON diet did not sort for or against short particles (<8, >4 mm; 100.2 ± 0.31%), but cows fed the LF diet tended to sort against these (99.2 ± 0.31%). Post-calving, cows fed the LF diet did not sort for long particles (98.5 ± 1.1%), but cows fed the CON diet continued to sort against these (96.3 ± 1.1%). Cows fed the LF diet had higher mean reticulorumen pH in the dry period (6.4 ± 0.05 vs. 6.2 ± 0.06 pH), in the first week after calving (CON = 5.7 ± 0.06 pH, LF = 5.8 ± 0.06 pH), and tended to have higher mean reticulorumen pH in the second week after calving (CON = 5.7 ± 0.06 pH, LF = 5.9 ± 0.06 pH). The results suggest that supplementing a molasses-based liquid feed in high-straw dry cow diets may improve intake and consistency in nutrients consumed during the dry period and in early lactation, as well as possibly promoting better rumen health across the transition period.

The objective of this study was to determine the effect of adding water to a high-straw dry cow diet on feeding behavior, health, and performance of dairy cows. Holstein cows (n = 40) entering their second or greater lactation were enrolled at dry off (~45 d before expected calving) and assigned to 1 of 2 dietary treatments, consisting of a high-straw dry cow total mixed ration [TMR; 36% wheat straw, 41% corn silage, 23% pellet, on a dry matter (DM) basis, formulated for 11.6% crude protein, 1.35 Mcal/kg net energy for lactation] with (1) no water (CON; n = 20; DM = 53.4%) or (2) water added to decrease the DM by ~10 percentage points (wet diet, WD; n = 20; DM = 45.4%). Upon calving, all cows were fed the same lactating TMR (45.2% DM, 14.9% crude protein, 1.68 Mcal/kg net energy for lactation) and followed for 28 d. Dry matter intake, feeding behavior, and rumination activity were recorded automatically. Fresh TMR and orts samples were collected 2×/wk to determine differences in sorting. A particle separator was used to separate feed samples into 4 fractions: long (>19 mm), medium (<19, >8 mm), short (<8, >4 mm), and fine (<4 mm) particles. Feed sorting was calculated as actual intake of each particle fraction expressed as a % of predicted intake. Cows fed WD had greater DM intake during the dry period than cows fed CON (CON = 13.4 ± 0.24, WD = 14.2 ± 0.24 kg/d), but rumination did not differ (CON = 522.2 ± 8.48, WD = 518.5 ± 8.69 min/d) between treatments. Cows fed WD tended to consume their feed faster (CON = 0.08 ± 0.001, WD = 0.09 ± 0.001 kg of DM/min) compared with CON cows. Regardless of treatment, cows sorted against the longest ration particles, with cows fed CON sorting more against these (CON = 81.1 ± 2.51%, WD = 94.9 ± 2.58%). Cows fed CON tended to sort more in favor of the medium particles than those fed WD (CON = 105.0 ± 0.36%, WD = 104.2 ± 0.37%). Postcalving DM intake and feeding behavior were not affected by dry diet treatment. Cows fed WD experienced a less rapid daily decline in rumen pH during the first 7 d after calving compared with those fed the CON diet. The results suggest that increasing the moisture content of high-straw dry cow diets, by adding water, may improve intake and reduce sorting against the longest ration particles, which may help promote consistency in targeted nutrients consumed during the dry period and greater rumen health in early lactation.

Body condition change during the dry period (ΔBCS) has been associated with a myriad of transition cow diseases. We used data from 3 studies to assess the relationship between ΔBCS, feeding behavior, and body condition score (BCS) at dry-off. We also studied the mediation effect that dry matter intake (DMI) has on ΔBCS and the association between dry period feeding time and DMI. A total of 100 parous cows were enrolled in 3 studies to investigate differences in dry period diet on behavior, health, and performance preand postcalving. Body weight was measured and BCS was assessed by the same trained observer after dry-off and 1 wk from calving date. The ΔBCS was calculated by subtracting the BCS at calving minus the BCS at dry-off. The BCS at dry-off was categorized as overconditioned (≥3.5) or not overconditioned (<3.5); no cows had a BCS <2.75. Feeding behavior data were collected using electronic feed bins. Parity at dry-off (median = 2; min = 1, max = 6) and 305-d milk production (mean = 10,235 kg, SD = 1,625 kg) from the previous lactation were considered. Data sets were split into 2 time periods: d −56 to −22 (early) and −21 to 0 (late) in relation to calving. Selected feeding behaviors (DMI, DMI as a percentage of body weight, and feeding time) were used to evaluate the associations between each feeding behavior and BCS at dry-off in each period using mixed linear regression models. Each model included the following covariates: parity, previous 305-d milk yield, and trial treatment. Experimental day was included as random slope, and cow was included as random intercept. A mediation analysis was used to evaluate the potential causal direct effect of BCS at dry-off on ΔBCS and the potential indirect effect mediated by differences in DMI. The BCS at dry-off was associated with changes in feeding behavior, such that overcon-ditioned cows had lesser daily DMI and feeding time during the early and late dry periods compared with not overconditioned animals. We also noted an effect of previous 305-d milk yield on DMI; cows that produced more milk had greater DMI throughout the dry period. The ΔBCS was only partially mediated by DMI, and BCS at dry-off still had a direct effect on ΔBCS. This result indicated that mechanisms other than DMI were associated with BCS loss during the dry period. Feeding time correlated weakly and moderately with DMI during the early and late dry periods, respectively. To conclude, strategies to improve intake during the dry period should take dry-off BCS into account or, preferably, efforts should be made to minimize the number of overconditioned cows at the end of lactation.