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SummaryAn improved method for the removal of polyphenolic compounds from aqueous extracts of plants is presented, The method removes >99% polyphenolic compounds from 5rag of extract. The method is simple, robust and reproducible. We examined the removal of polyphenolics from 5 different aqueous extracts of Chinese medicinal herbs.

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A microplate assay, for use with a variety of glycohydrolase enzymes, was developed to aid the screening of Chinese medicinal herb extracts for the presence of potential anti‐viral and anti‐lymphoma compounds. The microplate assay method described offers greater convenience, speed and reproducibility over existing methods. The enzymes tested were α‐glucosidase, β‐glucosidase and β‐glucuronidase. The assay can be easily adapted for use with other glycohydrolase enzymes. Of the 12 herb extracts examined four did not inhibit any of the enzymes (<50% inhibition), one inhibited α‐glucosidase only (>50% inhibition), six inhibited β‐glucuronidase only, and one inhibited both α‐glucosidase and β‐glucuronidase. None of the extracts were capable of inhibiting β‐glucosidase to any significant extent.

: HIV-1 integrase catalyzed the insertion of the viral DNA into the genome of human cells in the process of retrotranscription. Integrase is an attractive target for HIV-1 treatment due to the lack of its homologue in human cells and its vital role in HIV-1 replication. Although a major progress about the development of HIV-1 integrase inhibitors has been made, some thorny problems, such as the drug resistance, led to the further study of HIV-1 integrase inhibitors. This review briefly discussed the structure, function and mechanism of catalysis of HIV-1 integrase and made a further conclusion for recent advances in small-molecule inhibitors of HIV-1 integrase.