Removal of polyphenolic compounds from aqueous plant extracts using polyamide minicolumns

Collins, Richard A.; Ng, Tzi Bun; Fong, Wing‐Ping; Wan, C. C.; Yeung, H.W.

doi:10.1080/15216549800203212

Cited by 25 publications

(33 citation statements)

References 6 publications

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“…1). It is known that simple phenolic compounds with fewer phenolic hydroxyl groups bind less strongly to the polyamide resin, and can be eluted from the column (Collins et al, 1998). Therefore, it is speculated that there were still some tannins, such as gallic acid, present in the P. clypearia extract after treatment with the polyamide column as reported previously by Chen (1994).…”

Section: Resultsmentioning

confidence: 86%

“…1). In order to determine the influence of the tannins on the effect of the anti-HSV-1 activity of the herbs, these extracts were treated with a small polyamide column chromatography to remove them (Collins et al, 1998). It was found that the anti-HSV-1 activity of the aqueous extracts treated with the polyamide column was diminished dramatically.…”

Section: Resultsmentioning

confidence: 99%

“…A small polyamide column (Φ10 × 50 mm, 5 g, 100 mesh, Zhejiang, People's Republic of China) was used to remove the polyphenolic compounds from the aqueous herbal extracts (Collins et al, 1998). After the column was equilibrated with water, the aqueous extract was loaded on the column and allowed to percolate.…”

Section: Removal Of Polyphenols From Aqueous Extractsmentioning

confidence: 99%

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Antiviral activities of medicinal herbs traditionally used in southern mainland China

Ooi

Wang

et al. 2004

Phytotherapy Research

101

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The aqueous extracts from 21 medicinal herbs traditionally used in southern mainland China were screened for antiviral activities against human herpes simplex virus type 1 (HSV-1) and human respiratory syncytial virus (RSV) using a cytopathic effect (CPE) reduction assay. Three extracts from Agrimonia pilosa, Pithecellobium clypearia and Punica granatum, respectively, showed anti-HSV-1 activity, which was possibly contributed by the polyphenolic compounds in the herbal extracts. Six of the extracts, from Blumea laciniata, Elephantopus scaber, Laggera pterodonta, Mussaenda pubescens, Schefflera octophylla and Scutellaria indica, respectively, exhibited anti-RSV activity with 50% inhibition (IC50) concentrations ranging from 12.5 to 32 microg/mL, and selective indices (SI) ranging from 11.2 to 40. In addition to polyphenolic compounds, other constituents present in these extracts may also contribute to their anti-RSV activity.

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Section: Resultsmentioning

confidence: 86%

Section: Resultsmentioning

confidence: 99%

Section: Removal Of Polyphenols From Aqueous Extractsmentioning

confidence: 99%

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Antiviral activities of medicinal herbs traditionally used in southern mainland China

Ooi

Wang

et al. 2004

Phytotherapy Research

101

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“…Particularly common highly apolar compounds, such as fatty acids (Balunas et al, 2006), common polar compounds, such as polyphenols and flavonoids (Zhu et al, 1997(Zhu et al, , 2013Zou et al, 2002), as well as chlorophyll (Henrich et al, 2006) might be especially problematic since they can interfere with a range of different assays. Much effort is necessary to remove such constituents from samples prior to testing (Cardellina et al, 1993;Collins et al, 1998;Picker et al, 2014) or to modify the assay system in order to avoid their detection (Sasiela et al, 2008). Next to organic molecules, also some inorganic constituents, such as metals, can lead to false positive results in HTS (Hermann et al, 2013).…”

Section: Challenges Contributing To the Decline Of Plant-derived Natumentioning

confidence: 99%

Discovery and resupply of pharmacologically active plant-derived natural products: A review

2015

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Medicinal plants have historically proven their value as a source of molecules with therapeutic potential, and nowadays still represent an important pool for the identification of novel drug leads. In the past decades, pharmaceutical industry focused mainly on libraries of synthetic compounds as drug discovery source. They are comparably easy to produce and resupply, and demonstrate good compatibility with established high throughput screening (HTS) platforms. However, at the same time there has been a declining trend in the number of new drugs reaching the market, raising renewed scientific interest in drug discovery from natural sources, despite of its known challenges. In this survey, a brief outline of historical development is provided together with a comprehensive overview of used approaches and recent developments relevant to plant-derived natural product drug discovery. Associated challenges and major strengths of natural product- Europe PMC Funders Author ManuscriptsEurope PMC Funders Author Manuscripts based drug discovery are critically discussed. A snapshot of the advanced plant-derived natural products that are currently in actively recruiting clinical trials is also presented. Importantly, the transition of a natural compound from a "screening hit" through a "drug lead" to a "marketed drug" is associated with increasingly challenging demands for compound amount, which often cannot be met by re-isolation from the respective plant sources. In this regard, existing alternatives for resupply are also discussed, including different biotechnology approaches and total organic synthesis.While the intrinsic complexity of natural product-based drug discovery necessitates highly integrated interdisciplinary approaches, the reviewed scientific developments, recent technological advances, and research trends clearly indicate that natural products will be among the most important sources of new drugs also in the future.

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“…The column bound polyphenolic compounds were released by elution with 100 ml of absolute methanol (Collins, 1998).…”

Section: Organic Solvents and Column Partitioning Of Crude Extractsmentioning

confidence: 99%

Evaluation of retinoblastoma (Rb) and protein-53 (p53) gene expression levels in breast cancer cell lines (MCF-7) induced with some selected cytotoxic plants

Aderonke¹,

Ayorinde²,

Adesola³

et al. 2013

J. Pharmacognosy Phytother.

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Removal of polyphenolic compounds from aqueous plant extracts using polyamide minicolumns

Cited by 25 publications

References 6 publications

Antiviral activities of medicinal herbs traditionally used in southern mainland China

Antiviral activities of medicinal herbs traditionally used in southern mainland China

Discovery and resupply of pharmacologically active plant-derived natural products: A review

Evaluation of retinoblastoma (Rb) and protein-53 (p53) gene expression levels in breast cancer cell lines (MCF-7) induced with some selected cytotoxic plants

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