We used 3D cell printing to emulate an airway coupled with a naturally-derived blood vessel network in vitro. Decellularized extracellular matrix bioink derived from porcine tracheal mucosa (tmdECM) was used to encapsulate and print endothelial cells and fibroblasts within a designated polycarprolactone (PCL) frame. Providing a niche that emulates conditions in vivo, tmdECM gradually drives endothelial re-orientation, which leads to the formation of a lumen and blood vessel network. A fullydifferentiated in vitro airway model was assembled with the printed vascular platform, and collectively reproduced a functional interface between the airway epithelium and the vascular network. The model presented respiratory symptoms including asthmatic airway inflammation and allergen-induced asthma exacerbation in physiological context. Because of the adaptable and automated nature of direct 3D cell printing, we expect that this will have relevance in vivo and high reproducibility for production of high-content platforms for preclinical trials in biomedical research.
A novel hybrid construct was developed by combining aligned fibers (AFs) and random fibers (RFs) to form a scaffolding system. Homogeneous fiber-based structures were fabricated by electrospinning, which produced both random and aligned fiber mats depending on the collection method. The upper part of the scaffold contained an AF layer, which possessed a well-organized configuration that provided uniaxial topographic guidance. For mechanical stability and support, the lower part of the scaffold was composed of an RF layer. Despite the presence of randomly distributed RFs, desirable alignment and differentiation could be achieved in cultured C2C12 myoblasts by controlling the density of AF layer. The fibrous structure of the hybrid scaffold also exhibited high porosity and therefore reasonable permeability. Owing to the structural stability provided by the underlying RFs, the cell-laden fibrous scaffolds were amenable to physical manipulation, such as multilayering. Collectively, the morphological features and manipulable architecture of the developed scaffolds suggest that they would perform well in practical applications.
Building integrated photovoltaics (BIPVs) are attached to commercial and residential structures to enable solar energy harvesting. While conventional Si photovoltaics (PVs) are dominant in the current market, second and third...
In this review, we highlight the properties, functions and applications of stimuli-responsive hydrogel patterns in bioanalytical applications. Stimuli-responsive hydrogel patterns can be realized by well-established micro- and nanofabrication technologies such as photolithography and micromolding, and are currently adopted as active components for manipulation of flow and biosamples in microchannel and microarray systems. We overview the properties of stimuli-responsive hydrogel materials and their fabrication methods along with some representative examples in microfluidics and microarrays.
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