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Objectives:The aim of this study was to evaluate the sealing abilities of three different gutta-percha techniques in experimentally defective roots (EDR) and non-defective roots (NR).Materials and Methods:Sixty canine teeth were divided into six groups of ten; Group 1, NR + cold lateral condensation (CLC); Group 2, EDR + LC; Group 3, NR + BeeFill; Group 4, EDR + BeeFill; Group 5, NR + Thermafil; and Group 6, EDR + Thermafil. Apical leakage was measured using a computerized fluid filtration meter with a laser system.Results:Statistical analysis revealed that the CLC demonstrated more microleakage in the EDR than in the NR (P < 0.01). Thermafil demonstrated more microleakage in the NR than in the EDR (P < 0.01). No statistically significant differences were found between the BeeFill groups (P > 0.05).Conclusions:The results of this study indicate that internal resorptive cavities can affect the apical sealing properties of different root canal filling techniques, with Thermafil ensuring the lowest apical microleakage.

It is important to cover the pulp surface with a biocompatible material that is physically, mechanically, and chemically adequate. Graphene has the potential to form hard tissue, but at high doses, it shows toxic effects. It can be added to biocompatible materials at low doses to enhance their hard tissue forming potential. The aim of this study was to compare the physical, chemical, and mechanical properties of graphene nanoplatelet (GNP) added Angelus mineral trioxide aggregate (A‐MTA) to pure A‐MTA and calcium hydroxide. Homogeneous mixtures (created by adding +0.1 weight[wt]% and 0.3 wt% GNP to A‐MTA), pure A‐MTA, and Dycal were used. Three disc‐shaped samples of each material were prepared using Teflon mold. Scanning electron microscope–energy dispersive X‐ray (SEM–EDX), particle size, microhardness, and Fourier transform infrared spectroscopy (FTIR) analysis of the materials were performed in vitro. Data were analyzed using Kruskal–Wallis test followed by Conover test (p < .001). A‐MTA and GNP added samples showed similar peaks in FTIR analysis. In the EDX analysis, the amount of carbon was observed with a higher increase at A‐MTA + 0.3 wt% GNP than A‐MTA + 0.1 wt% GNP. In the SEM image, hollow structure and particle size decreased as the amount of GNP increased; particle size was smaller at A‐MTA + 0.3 wt% GNP than A‐MTA + 0.1 wt% GNP (p < .001). A‐MTA + 0.3 wt% GNP showed the highest microhardness while Dycal showed the lowest microhardness. The addition of GNP, a material with high potential for forming hard tissue, to the structure of capping materials can also positively contribute to the microhardness of the capping materials.

PurposeThis study aimed to compare the effects of the collagen-BioAggregate mixture (CBA-M) and collagen-BioAggregate composite (CBA-C) sponge as a scaffolding material on the reparative dentin formation. Materials and MethodsCBA-C sponge (10:1 w/w) was obtained and characterized by Scanning Electron Microscopy (SEM) and Mercury Porosimetry. Cytotoxicity of the CBA-C sponge was tested by using the L929 mouse fibroblast cell line. Dental pulp stem cells (DPSCs) were isolated from the pulp tissue of sheep teeth and characterized by flow cytometry for the presence of mesenchymal stem cell marker, CD44. The osteogenic differentiation capability of isolated DPSCs was studied by Alizarin Red staining. The cells were then used to study for the compatibility of CBA-C sponge with cell proliferation and calcium phosphate deposition. The effect of CBA-C sponge and CBA-M on the induction of dentin regeneration was studied in the perforated teeth of sheep for the eight-week period. All the analyses were performed with appropriate statistical hypothesis tests. ResultsCBA-C sponge was found to be biocompatible for DPSCs. The DPSCs seeded on the CBA-C sponge were able to differentiate into the osteoblastic lineage and deposit calcium phosphate crystals in vitro. Reparative dentin formation was observed after the second week in the CBA-C sponge applied group. At the end of eight weeks, a complete reparative dentin structure was formed in the CBA-C sponge applied group, whereas necrotic tissue residues were observed in groups treated with the CBA-M. ConclusionCBA-C sponge represents a better microenvironment for reparative dentin formation probably due to maintaining DPSCs and allowing their osteogenic differentiation and thus calcium phosphate deposition.

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Aim: Recently, although studies have shown that biomaterials containing graphene oxide (GO) in biomedicine stand out for their positive effects, the effect of GO on dental tissues when used with dental materials is not well known. The aim of this study was an evaluation of the cytotoxic effects of GO on gingival fibroblasts when it is combined in two different ratios with Mineral Trioxide Aggregate (MTA). Materials and Methods: In this in-vitro study, a homogenous mixture of adding +0.1 weight (wt)% and +0.3 wt% GO to Angelus MTA was created (two experimental groups) and compared with pure Angelus MTA and negative control groups. The materials were mixed according to the manufacturer’s instructions, and Teflon molds were used to form 24 disc-shaped samples for each group. The samples were divided into groups according to the simple random sampling method. The cytotoxic effect of samples was determined on gingival fibroblast cells by using the MTT test, and total oxidant status (TOS) and total antioxidant capacity (TAC) kits in 24 and 72 hours. The data were statistically analyzed using one-way ANOVA and Tukey tests. Results: A significant difference was found between the material-applied groups and the control group at the TAC 24 and 72 hours and between the groups containing GO and the control group at the MTT 72 hours and TAC and TOS 24 and 72 hours ( p < .05). Conclusion: The addition of GO to MTA increased the dose and time-based toxicity and oxidant amount, and decreased antioxidant capacity.

Objective: Obesity is a global, chronic disease that affects both developed and developing countries. The purpose of this study is to examine the effect of morbid obesity and obesity affect the decayed, missing, and filled teeth (DMFT) index and saliva values. Methods: The study included 50 morbidly obese, 50 obese, and 50 control group patients ranging in age from 18 to 68. The DMFT index of the patients was determined by clinical examination. The saliva kit was used to determine saliva values [unstimulated saliva flow rate (USFR), stimulated saliva flow rate (SSFR), saliva viscosity (SV), saliva pH (SpH), and saliva buffering capacity (SBC)]. The patients were surveyed to learn about their oral health habits, nutritional habits, and socioeconomic status. Results: There was no significant difference in the number of decayed teeth, filled teeth, USFR, or SV between the groups (p>0.05). The DMFT index, number of missing teeth, SSFR, SpH, and SBC all showed significant differences between the groups (p

Amaç: Bu in vitro çalışmanın amacı mikrohibrit, submikrohibrit ve bulkfill kompozitin kompozit tamir materyali olarak mikro gerilme bağlanma dayanımının ve kırılma yüzeylerindeki başarısızlık tipinin karşılaştırmalı olarak değerlendirilmesidir. Gereç ve Yöntem: Standart Teflon kalıplarda supranano kompozit rezinden (Estelite Sigma Quick) silindirik (4x4 mm) örnekler hazırlandı ve 3.000 termal döngüye maruz bırakıldı. Örneklerin bir yüzeyi abraze hale getirilerek mikrohibrit kompozit (Arabesk), submikrohibrit kompozit (Charisma Smart) ve bulkfill kompozit (Filtek Bulk-Fill Posterior) ile restore edilip 4x8 mm boyutlarında bloklar oluşturuldu. Kompozit bloklardan kesme cihazı ile her grup için 12 adet olmak üzere 1x1x8 mm3 ölçülerinde dikdörtgenler prizması şeklinde çubuklar elde edilerek mikro gerilim test cihazı ile bağlanma dayanımları test edildi. Ayrıca test sonrası her örneğin kopma yüzeyi stereo mikroskopta incelendi. Tamir bağlanma dayanımı verileri tek yönlü varyans analizi (ANOVA) ile karşılaştırıldı ve çoklu karşılaştırmalar Tukey HSD testi ile değerlendirildi. Bütün pMikrohibrit). Sonuç: Supranano kompozit rezinin tamirinde submikrohibrit ve mikrohibrit kompozite göre bulkfill kompozit ile daha iyi bir bağlanma sağlanabilir.