Evaluation of a collagen-bioaggregate composite scaffold in the repair of sheep pulp tissue

Dayi, Burak; Bilecen, Deniz; Eroksuz, Hatice; Yalçın, Muhammet; Hasırcı, Vasıf

doi:10.26650/eor.2021911441

Cited by 4 publications

(1 citation statement)

References 40 publications

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“…Collagen has high biocompatibility and biological activity, and it can promote cell adhesion, migration, and proliferation [ 218 ]. Collagen has poor mechanical properties but is adequate for RET, and its mechanical properties can be enhanced through crosslinking with other elements or constructing composite scaffolds [ 219 ]. Collagen can carry pulp stem/progenitor cells with or without GFs and anti-inflammatory molecules to form pulp-like tissue in vivo [ 31 , 33 , 35 , 36 , 37 , 38 , 39 , 40 , 41 ].…”

Section: The Role Of Scaffolds In Promoting Neural Regenerationmentioning

confidence: 99%

Neural Regeneration in Regenerative Endodontic Treatment: An Overview and Current Trends

Wei

Lyu

et al. 2022

IJMS

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Pulpal and periapical diseases are the most common dental diseases. The traditional treatment is root canal therapy, which achieves satisfactory therapeutic outcomes—especially for mature permanent teeth. Apexification, pulpotomy, and pulp revascularization are common techniques used for immature permanent teeth to accelerate the development of the root. However, there are obstacles to achieving functional pulp regeneration. Recently, two methods have been proposed based on tissue engineering: stem cell transplantation, and cell homing. One of the goals of functional pulp regeneration is to achieve innervation. Nerves play a vital role in dentin formation, nutrition, sensation, and defense in the pulp. Successful neural regeneration faces tough challenges in both animal studies and clinical trials. Investigation of the regeneration and repair of the nerves in the pulp has become a serious undertaking. In this review, we summarize the current understanding of the key stem cells, signaling molecules, and biomaterials that could promote neural regeneration as part of pulp regeneration. We also discuss the challenges in preclinical or clinical neural regeneration applications to guide deep research in the future.

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Section: The Role Of Scaffolds In Promoting Neural Regenerationmentioning

confidence: 99%

Neural Regeneration in Regenerative Endodontic Treatment: An Overview and Current Trends

Wei

Lyu

et al. 2022

IJMS

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Elderberry (Sambucus nigra) and hawthorn (Crataegus oxyacantha) extract additives in carboxymethyl chitosan scaffolds for osteochondral tissue engineering applications

Baysan,

Yilmaz,

Husemoglu

et al. 2024

J of Applied Polymer Sci

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Advancements in new treatment methods are becoming increasingly necessary due to a longer life expectancy, particularly in the field of osteochondral tissue engineering. In the present study, osteochondral tissue scaffolds with/without the addition of hawthorn or elderberry extracts were fabricated. Poly(ethylene glycol) diglycidyl ether (PEGDE) cross‐linked carboxymethyl chitosan hydrogel scaffold matrix was used as a control group. The addition of the plant extracts resulted in open porous structures with pore sizes around 100–450 μm and elastic modulus between 0.5 and 0.7 MPa, biomimicking the osteochondral tissue. Scaffolds containing either plant extract showed a high swelling tendency (1500–1750%) and a delayed release of the extracts after a week. Furthermore, the weight loss of the scaffolds after an enzymatic degradation for 56 days was ~30%. In addition, in vitro biocompatibility analyses were performed using bone marrow‐derived mesenchymal stem cells confirming non‐cytotoxicity for all the scaffold types. Biochemical assessment of alkaline phosphatase activity and glycosaminoglycan content yielded results consistent with osteochondral scaffold requirements. Finally, immunohistochemical analyses indicated that the plant extract addition, especially elderberry, increased collagen type I and type II formation. As a result, the addition of both hawthorn and elderberry extracts is a promising approach to osteochondral tissue regeneration.

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Loofah and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) nano-fiber-reinforced chitosan hydrogel composite scaffolds with elderberry (Sambucus nigra) and hawthorn (Crataegus oxyacantha) extracts as additives for osteochondral tissue engineering applications

Baysan,

Yilmaz,

Albayrak

et al. 2024

Polym. Bull.

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In recent years, people have had more expectations from the developed technology in medicine, especially in the field of orthopedics and traumatology. Tissue engineers are interested in techniques that benefit from patients’ cells and biomaterials, instead of prostheses and implants. On the other hand, researchers have begun to use various medicinal plants for regeneration and anti-cancer studies. In the present study, we aimed to produce cartilage and bone inductive scaffolds for osteochondral tissue engineering applications with the addition of hawthorn or elderberry extracts. Firstly, wet electro-spun poly (3-hydroxybutyric acid-co-3-hydroxyvaleric acid) fibers were integrated with a loofah mat. Then, they were impregnated into chitosan solution with/without hawthorn or elderberry extract. Composite hydrogel scaffolds were obtained by cross-linking with 0.3% (w/v) genipin. Fabricated scaffolds had more than 90% porosity and showed swelling capacity in the range of 1500–2200%. Based on the in vitro biocompatibility analyses using mesenchymal stem cells (MSCs), all the fabricated scaffolds were found to be biocompatible by WST-1, ALP activity, and GAG content analysis. Also, histological/immunohistochemical analyses showed that hawthorn and elderberry extract addition increased MSCs proliferation and collagen type I and II positivity. Consequently, all the scaffolds showed promising features for osteochondral tissue engineering applications. Graphical abstract

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Evaluation of a collagen-bioaggregate composite scaffold in the repair of sheep pulp tissue

Cited by 4 publications

References 40 publications

Neural Regeneration in Regenerative Endodontic Treatment: An Overview and Current Trends

Neural Regeneration in Regenerative Endodontic Treatment: An Overview and Current Trends

Elderberry (Sambucus nigra) and hawthorn (Crataegus oxyacantha) extract additives in carboxymethyl chitosan scaffolds for osteochondral tissue engineering applications

Loofah and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) nano-fiber-reinforced chitosan hydrogel composite scaffolds with elderberry (Sambucus nigra) and hawthorn (Crataegus oxyacantha) extracts as additives for osteochondral tissue engineering applications

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